Evidence map›Paper›PMID 35727016›Full record

ArticlemSphere2022

Turnover of Variant Surface Glycoprotein in Trypanosoma brucei Is Not Altered in Response to Specific Silencing.

Mohamed Sharif, Paige Garrison, Peter Bush, James D Bangs

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. The endoplasmic reticulum of trypanosomatids: An unrevealed road for chemotherapy.Frontiers in cellular and infection microbiology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Mohamed Sharif *Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), Buffalo, New York, USA.
Paige Garrison *Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), Buffalo, New York, USA.
Peter BushSouth Campus Instrument Center, School of Dental Medicine, University at Buffalo (SUNY), Buffalo, New York, USA.
James D BangsDepartment of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo (SUNY), Buffalo, New York, USA.ORCID 0000-0001-7903-6521
University at Buffalo, State University of New York · US

Funding

Secretory Protein Trafficking and Folding in African TrypanosomesR01AI035739 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BANGS, JAMES D. · 2000 to 2020
$6.6M
SECRETION AND PROTEIN TARGETING IN TRYPANOSOMA BRUCEIR29AI035739 · NIAID · UNIVERSITY OF WISCONSIN MADISON · PI BANGS, JAMES D. · 1995 to 1999
–
NIAID NIH HHS R01 AI035739NIAID NIH HHS R29 AI035739
6 · The paper itself

Abstract

African trypanosomes evade the immune system of the mammalian host by the antigenic variation of the predominant glycosylphosphatidylinositol (GPI)-anchored surface protein, variant surface glycoprotein (VSG). VSG is a very stable protein that is turned over from the cell surface with a long half-life (~26 h), allowing newly synthesized VSG to populate the surface. We have recently demonstrated that VSG turnover under normal growth is mediated by a combination of GPI hydrolysis and direct shedding with intact GPI anchors. VSG synthesis is tightly regulated in dividing trypanosomes, and when subjected to RNA interference (RNAi) silencing, cells display rapid cell cycle arrest in order to conserve VSG density on the cell surface (K. Sheader, S. Vaughan, J. Minchin, K. Hughes, et al., Proc Natl Acad Sci U S A 102:8716-8721, 2005, https://doi.org/10.1073/pnas.0501886102). Arrested cells also display an altered morphology of secretory organelles-engorgement of the

Indexed as

Trypanosoma brucei bruceiAnimalsAntigenic VariationGlycosylphosphatidylinositolsHumansMammalsMembrane GlycoproteinsVariant Surface Glycoproteins, TrypanosomaGlycosylphosphatidylinositolsMembrane GlycoproteinsVariant Surface Glycoproteins, Trypanosomaglycosylphosphatidylinositolprotein turnoverRNAi silencingtrypanosomevariant surface glycoprotein

Identifiers

PMID35727016
PMCPMC9429888
OpenAlexW4283215406

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.