ArticlemBio2022
Microbiome Analysis for Wastewater Surveillance during COVID-19.
Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Wastewater-Based Surveillance of Human Papillomavirus Genotype Diversity.JAMA network open · 2026Article
- Article
- From plant pathogen to public health tool: A review of tobamoviruses and their role in wastewater & environmental surveillance.Journal of water and health · 2026Review
- Article
- High prevalence of tomato brown rugose fruit virus in domestic wastewater as a potential viral indicator for treatment systems.Scientific reports · 2026Article
- Pathogen-Phage Geomapping to Overcome Resistance.bioRxiv : the preprint server for biology · 2025Article
- Hybridization capture sequencing formBio · 2025Article
- Identification of circulating human papillomavirus types through high-throughput sequencing of Canadian municipal and institutional wastewater samples.Applied and environmental microbiology · 2025Article
- Wastewater Metavirome Diversity: Exploring Replicate Inconsistencies and Bioinformatic Tool Disparities.International journal of environmental research and public health · 2025Article
- Interpretation of indoor air surveillance for respiratory infections: a prospective longitudinal observational study in a childcare setting.EBioMedicine · 2025Observational
- Culture-Dependent and -Independent Wastewater Surveillance for Multiple Pathogenic Yeasts.Journal of fungi (Basel, Switzerland) · 2025Article
- Metagenomic insights into the urban-rural variation of antimicrobial resistance and pathogen reservoirs in untreated wastewater from central India.Frontiers in microbiology · 2025Article
- Article
- Microbial diversity, genomics, and phage-host interactions of cyanobacterial harmful algal blooms.mSystems · 2024Article
- Saliva-based detection of SARS-CoV-2: a bibliometric analysis of global research.Molecular and cellular biochemistry · 2024Article
- Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission.iScience · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Building Environmental and Sociological Predictive Intelligence to Understand the Seasonal Threat of SARS-CoV-2 in Human Populations.The American journal of tropical medicine and hygiene · 2024Article
- Metabolites: a converging node of host and microbe to explain meta-organism.Frontiers in microbiology · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 5 institutions in 1 country.
Funding
Abstract
Wastewater surveillance (WS), when coupled with advanced molecular techniques, offers near real-time monitoring of community-wide transmission of SARS-CoV-2 and allows assessing and mitigating COVID-19 outbreaks, by evaluating the total microbial assemblage in a community. Composite wastewater samples (24 h) were collected weekly from a manhole between December 2020 and November 2021 in Maryland, USA. RT-qPCR results showed concentrations of SARS-CoV-2 RNA recovered from wastewater samples reflected incidence of COVID-19 cases. When a drastic increase in COVID-19 was detected in February 2021, samples were selected for microbiome analysis (DNA metagenomics, RNA metatranscriptomics, and targeted SARS-CoV-2 sequencing). Targeted SARS-CoV-2 sequencing allowed for detection of important genetic mutations, such as spike: K417N, D614G, P681H, T716I, S982A, and D1118H, commonly associated with increased cell entry and reinfection. Microbiome analysis (DNA and RNA) provided important insight with respect to human health-related factors, including detection of pathogens and their virulence/antibiotic resistance genes. Specific microbial species comprising the wastewater microbiome correlated with incidence of SARS-CoV-2 RNA, suggesting potential association with SARS-CoV-2 infection. Climatic conditions, namely, temperature, were related to incidence of COVID-19 and detection of SARS-CoV-2 in wastewater, having been monitored as part of an environmental risk score assessment carried out in this study. In summary, the wastewater microbiome provides useful public health information, and hence, a valuable tool to proactively detect and characterize pathogenic agents circulating in a community. In effect, metagenomics of wastewater can serve as an early warning system for communicable diseases, by providing a larger source of information for health departments and public officials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.