ArticleNature biotechnology2022
Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds.
Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 86 citations in OpenAlex.
- Stepwise Conformational Restriction of Acylhydrazone-Based Molecules for High Signal-to-Noise Fluorescent Recognition of Synthetic Cannabinoid FUB-INACA.Angewandte Chemie (International ed. in English) · 2026Article
- Protein Engineering of a Genetically Encodable Biosensor for Wastewater Detection of Profen NSAIDs.Biotechnology and bioengineering · 2026Article
- The Fragmented Nature of Biosensor Development: Challenges and Paths to Mitigation.Biosensors · 2026Review
- Phytohormone dynamics in tomato leaves due to in-situ continuous wearing of sensors.Plant methods · 2026Article
- The Promise of Synthetic Biology for Redesigning Plant Architecture.International journal of molecular sciences · 2026Review
- A single-cell screening platform accelerates functional genetics in plants.Nature biotechnology · 2026Article
- The genetic architecture of an allosteric hormone receptor.Nature communications · 2026Article
- Review
- Small-molecule binding and sensing with a designed protein family.Nature communications · 2026Article
- Repurposing nuclear receptors for ligand-responsive liquid condensate formation and gene regulation.Nature communications · 2026Article
- Protein engineering of a genetically encoded biosensor for wastewater detection of profen NSAIDs.bioRxiv : the preprint server for biology · 2026Article
- Computational redesign of a thermostable T7 RNA polymerase.Protein engineering, design & selection : PEDS · 2026Article
- Computational design of dynamic biosensors for emerging synthetic opioids.Nature communications · 2026Article
- Unusually broad-spectrum small-molecule sensing using a single protein scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Computational redesign of a thermostable T7 RNA polymerase.bioRxiv : the preprint server for biology · 2025Article
- PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production inACS synthetic biology · 2025Article
- High-Throughput Fluorescence Screening Enables Globally Consistent Identification of ABA Signaling Modulators.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Computational design of dynamic biosensors for emerging synthetic opioids.bioRxiv : the preprint server for biology · 2025Article
- Bacterial network for precise plant stress detection and enhanced crop resilience.BMC bioinformatics · 2025Article
- Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.Nature nanotechnology · 2025Article
Corrections and comments
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Authors and funding
19 authors at 5 institutions in 1 country.
Funding
Abstract
A general method to generate biosensors for user-defined molecules could provide detection tools for a wide range of biological applications. Here, we describe an approach for the rapid engineering of biosensors using PYR1 (Pyrabactin Resistance 1), a plant abscisic acid (ABA) receptor with a malleable ligand-binding pocket and a requirement for ligand-induced heterodimerization, which facilitates the construction of sense-response functions. We applied this platform to evolve 21 sensors with nanomolar to micromolar sensitivities for a range of small molecules, including structurally diverse natural and synthetic cannabinoids and several organophosphates. X-ray crystallography analysis revealed the mechanistic basis for new ligand recognition by an evolved cannabinoid receptor. We demonstrate that PYR1-derived receptors are readily ported to various ligand-responsive outputs, including enzyme-linked immunosorbent assay (ELISA)-like assays, luminescence by protein-fragment complementation and transcriptional circuits, all with picomolar to nanomolar sensitivity. PYR1 provides a scaffold for rapidly evolving new biosensors for diverse sense-response applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.