Evidence map›Paper›PMID 35725373›Full record

ArticleBMC genomics2022

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus).

Zhi He, Lijuan Ye, Deying Yang, Zhijun Ma, Faqiang Deng, Zhide He, Jiaxiang Hu, Hongjun Chen, Li Zheng, Yong Pu and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
4.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 2 institutions in 1 country.

Zhi He *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Lijuan Ye *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Deying Yang *College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhijun MaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Faqiang DengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhide HeLuzhou Municipal Bureau of Agriculture and Rural Affairs, Luzhou, 646000, Sichuan, China.
Jiaxiang HuSichuan Water Conservancy Vocational College, Chengdu, 611231, Sichuan, China.
Hongjun ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Li ZhengCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yong PuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yuanyuan JiaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qiqi ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Kuo GaoCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jinxin XiongCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Bolin LaiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaobin GuCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xiaoli HuangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Shiyong YangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Mingwang ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Taiming YanCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. yantaiming@sicau.edu.cn.
Sichuan Agricultural University · CNHealth and Family Planning Commission of Sichuan Province · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAn increasing number of long noncoding RNAs (lncRNAs) have been found to play important roles in sex differentiation and gonad development by regulating gene expression at the epigenetic, transcriptional and posttranscriptional levels. The ricefield eel, Monopterus albus, is a protogynous hermaphroditic fish that undergoes a sequential sex change from female to male. However, the roles of lncRNA in the sex change is unclear.

resultsHerein, we performed RNA sequencing to analyse lncRNA expression patterns in five different stages of M. albus development to investigate the roles of lncRNAs in the sex change process. A total of 12,746 lncRNAs (1503 known lncRNAs and 11,243 new lncRNAs) and 2901 differentially expressed lncRNAs (DE-lncRNAs) were identified in the gonads. The target genes of the DE-lncRNAs included foxo1, foxm1, smad3, foxr1, camk4, ar and tgfb3, which were mainly enriched in signalling pathways related to gonadal development, such as the insulin signalling pathway, MAPK signalling pathway, and calcium signalling pathway. We selected 5 highly expressed DE-lncRNAs (LOC109952131, LOC109953466, LOC109954337, LOC109954360 and LOC109958454) for full length amplification and expression pattern verification. They were all expressed at higher levels in ovaries and intersex gonads than in testes, and exhibited specific time-dependent expression in ovarian tissue incubated with follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG). The results of quantitative real-time PCR (qRT-PCR) analysis and a dual-luciferase assay showed that znf207, as the gene targeted by LOC109958454, was expressed in multiple tissues and gonadal developmental stages of M. albus, and its expression was also inhibited by the hormones FSH and hCG.

conclusionsThese results provide new insights into the role of lncRNAs in gonad development, especially regarding natural sex changes in fish, which will be useful for enhancing our understanding of sequential hermaphroditism and sex changes in the ricefield eel (M. albus) and other teleosts.

Indexed as

Disorders of Sex DevelopmentRNA, Long NoncodingSmegmamorphaAnimalsEelsFemaleFollicle Stimulating HormoneGonadsMaleFollicle Stimulating HormoneRNA, Long NoncodingGonadal developmentlncRNAMonopterus albusRNA-seq

Identifiers

PMID35725373
PMCPMC9208217
OpenAlexW4283168337

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LicenceCC BY
Read underepoch 390

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.