Evidence map›Paper›PMID 35724830›Full record

ArticleMicrobial pathogenesis2022

Contribution of Streptococcus pyogenes M87 protein to innate immune resistance and virulence.

Yujiro Hirose, Piotr Kolesinski, Masanobu Hiraoka, Satoshi Uchiyama, Raymond H Zurich, Monika Kumaraswamy, Elisabet Bjanes, Partho Ghosh, Shigetada Kawabata, Victor Nizet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial pathogenesis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Antibiotics (Basel, Switzerland) · 2023
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yujiro HiroseDepartment of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka, 5650871, Japan; Department of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA. Electronic address: yujirohirose@dent.osaka-u.ac.jp.
Piotr KolesinskiDepartment of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, 92093, USA.
Masanobu HiraokaDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA; Department of Otorhinolaryngology-Head and Neck Surgery, Wakayama Medical University, Wakayama, Wakayama, 6418509, Japan.
Satoshi UchiyamaDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA.
Raymond H ZurichDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA.
Monika KumaraswamyDepartment of Medicine, University of California at San Diego, La Jolla, CA, 92093, USA; Infectious Diseases Section, VA San Diego Healthcare System, San Diego, CA, 92161, USA.
Elisabet BjanesDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA.
Partho GhoshDepartment of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA, 92093, USA.
Shigetada KawabataDepartment of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry, Suita, Osaka, 5650871, Japan. Electronic address: kawabata@dent.osaka-u.ac.jp.
Victor NizetDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, 92093, USA; Skaggs School of Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA, 92093, USA.

Funding

Cathelicidins in Skin ImmunityR01AI052453 · NIAID · VETERANS MEDICAL RESEARCH FDN/SAN DIEGO · PI GALLO, RICHARD L, NIZET, VICTOR · 2002 to 2017
$5.3M
Evasion of host immunity by the M proteinR01AI154149 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, PARTHO · 2020 to 2024
$3.2M
C3-Dependent Intracellular Killing in Innate Immunity and Bacterial PathogenesisR01AI145325 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NIZET, VICTOR, RAFFATELLU, MANUELA · 2019 to 2023
$3.1M
Mechanisms of the M protein in group A Streptococcus virulenceR01AI096837 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, PARTHO, NIZET, VICTOR · 2011 to 2015
$2.7M
How group A Streptococcus M1 protein resists cathelicidinsR21AI144901 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, PARTHO · 2019 to 2020
$433k
NIAID NIH HHS R01 AI052453NIAID NIH HHS R01 AI096837NIAID NIH HHS R01 AI145325NIAID NIH HHS R01 AI154149NIAID NIH HHS R21 AI144901
6 · The paper itself

Abstract

Streptococcus pyogenes is a pre-eminent human pathogen, and classified by the hypervariable sequence of the emm gene encoding the cell surface M protein. Among a diversity of M/emm types, the prevalence of the M/emm87 strain has been steadily increasing in invasive S. pyogenes infections. Although M protein is the major virulence factor for globally disseminated M/emm1 strain, it is unclear if or how the corresponding M protein of M/emm87 strain (M87 protein) functions as a virulence factor. Here, we use targeted mutagenesis to show that the M87 protein contributes to bacterial resistance to neutrophil and whole blood killing and promotes the release of mature IL-1β from macrophages. While deletion of emm87 did not influence epithelial cell adherence and nasal colonization, it significantly reduced S. pyogenes-induced mortality and bacterial loads in a murine systemic infection model. Our data suggest that emm87 is involved in pathogenesis by modulating the interaction between S. pyogenes and innate immune cells.

Indexed as

Streptococcal InfectionsStreptococcus pyogenesAnimalsAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsHumansImmunity, InnateMiceVirulenceVirulence FactorsAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsVirulence Factorsemm87M proteinStreptococcus pyogenesVirulence factor

Identifiers

PMID35724830
PMCPMC9878354

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.