Evidence map›Paper›PMID 35723374›Full record

ArticleCurrent issues in molecular biology2022

Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair.

Wanqing Xu, Qingxia Zuo, Dongyan Feng, Changsheng He, Cailing Lin, Dongchao Huang, Yanbin Wan, Feng Chen, Guosheng Mo, Qi Sun and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Wanqing XuSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Qingxia ZuoSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Dongyan FengSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Changsheng HeSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Cailing LinSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Dongchao HuangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Yanbin WanSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Feng ChenSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Guosheng MoSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Qi SunSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Hongli DuSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
Lizhen HuangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.ORCID 0000-0002-9839-4620
South China University of Technology · CN

Funding

Fundamental Research Funds for the Central Universities from South China University of Technology 2019MS089Medical Scientific Research Foundation of Guangdong Province of China A2019432National Natural Science Foundation of China 31871292Natural Science Foundation of Guangdong Province of China 2019A1515010619The National Key R&D Program of China 2018YFC0910201
6 · The paper itself

Abstract

An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. Herein, we tested the utility of the widespread promoterless EGFP reporter to assess the efficiency of CRISPR/Cas9-mediated homologous recombination by fluorescence expression. We firstly established a promoterless EGFP reporter donor targeting the porcine

Indexed as

CRISPR/Cas9homology-directed repairprecise gene editingpromoterless EGFP reporterrandom integration

Identifiers

PMID35723374
PMCPMC9164083
OpenAlexW4223599647

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.