ArticleSTAR protocols2022
Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis.
Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 9 citations in OpenAlex.
- MEIOSIN retains a STRA8-independent activity that contributes to meiotic gene activation across vertebrates.Genes & development · 2026Article
- Mettl1-dependent mNature communications · 2024Article
- Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions.Nature communications · 2024Article
- STRA8-RB interaction is required for timely entry of meiosis in mouse female germ cells.Nature communications · 2023Article
- HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation.Nature communications · 2023Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest tagged with 3xFLAG-HA in the testis. The protocol is optimized for salt extraction of cytoplasmic and nuclear proteins from mouse frozen testes and thus can be used for a variety of proteins. For complete details on the use and execution of this protocol, please refer to Ishiguro et al. (2020) and Tanno et al. (2022).
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Registered trials
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