ArticleScientific reports2022
Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Understanding and targeting the tumour matrisome.Nature reviews. Clinical oncology · 2026Review
- Double Stokes polarimetry of birefringent structures.Biomedical optics express · 2026Article
- Article
- Label-free histological identification of intraductal carcinoma of the prostate using texture analysis-based multimodal stimulated Raman scattering microscopy.Scientific reports · 2025Article
- Polarimetric feature analysis of Mueller matrices for brain tumor image segmentation.Optics express · 2025Article
- Second harmonic generation for brain imaging: pathology-related studies.Biophysical reviews · 2025Article
- Polar organization of H&E dyes in histology tissue revealed by polarimetric nonlinear microscopy.Biomedical optics express · 2025Article
- Double Stokes polarimetric microscopy for chiral fibrillar aggregates.Scientific reports · 2025Article
- Emerging research trends in artificial intelligence for cancer diagnostic systems: A comprehensive review.Heliyon · 2024Review
- Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion.Biomedical optics express · 2024Article
- Imaging of colorectal adenomas with pseudoinvasion and malignant polyps using two-photon excitation microscopy.Frontiers in oncology · 2024Article
- Polarimetric second harmonic generation microscopy of partially oriented fibers II: Imaging study.Biophysical journal · 2023Article
- High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.Nanophotonics (Berlin, Germany) · 2023Article
- Nonlinear microscopy and deep learning classification for mammary gland microenvironment studies.Biomedical optics express · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Unsupervised determination of lung tumor margin with widefield polarimetric second-harmonic generation microscopy.Scientific reports · 2022Article
- Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The extracellular matrix (ECM) collagen undergoes major remodeling during tumorigenesis. However, alterations to the ECM are not widely considered in cancer diagnostics, due to mostly uniform appearance of collagen fibers in white light images of hematoxylin and eosin-stained (H&E) tissue sections. Polarimetric second-harmonic generation (P-SHG) microscopy enables label-free visualization and ultrastructural investigation of non-centrosymmetric molecules, which, when combined with texture analysis, provides multiparameter characterization of tissue collagen. This paper demonstrates whole slide imaging of breast tissue microarrays using high-throughput widefield P-SHG microscopy. The resulting P-SHG parameters are used in classification to differentiate tumor from normal tissue, resulting in 94.2% for both accuracy and F1-score, and 6.3% false discovery rate. Subsequently, the trained classifier is employed to predict tumor tissue with 91.3% accuracy, 90.7% F1-score, and 13.8% false omission rate. As such, we show that widefield P-SHG microscopy reveals collagen ultrastructure over large tissue regions and can be utilized as a sensitive biomarker for cancer diagnostics and prognostics studies.
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