Evidence map›Paper›PMID 35717235›Full record

ArticlePlant methods2022

Construction of the SHP-GLOX lignin regulation system and its application in rice straw.

Qingdong Wang, Jiayuan Zhang, Yan Li, Ran Wang

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Qingdong WangHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Jiayuan ZhangHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Yan LiHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Ran WangHenan Key Laboratory of Bioactive Macromolecules, Laboratory of Straw Enzymatic Technology Research, College of Life Science, Zhengzhou University, Zhengzhou, 450001, Henan, China. wangran@henau.edu.cn.
Zhengzhou University · CN

Funding

National Natural Science Foundation of China 51508518Special Fund for Agroscientific Research 201503134
6 · The paper itself

Abstract

backgroundThere is great productivity of rice(Oryza sativa L. spp. japonica) straw in China, which is a potential source of biomass for biofuel and forage. However, the high levels of lignins in rice straw limited its usage and induced the formation of agricultural waste. In order to modify the lignins contents to improve biofuel production and forage digestibility, we selected Soybean hull peroxidase (SHP) and Glyoxal oxidase (GLOX) as candidate genes to improve quality of rice straw. SHP, a class III plant peroxidase, is derived from multiple sources. It has several advantages, such as high resistance to heat, high stability under acidic and alkaline conditions, and a broad substrate range. SHP is speculated to be useful for lignin degradation. Glyoxal oxidase (GLOX) is an extracellular oxidase that can oxidize glyoxal and methylglyoxal in the extracellular medium to generate H

resultsIn the present study, the SHP and GLOX genes in pCAMBIA3301-glycine-rich protein (GRP)-SHP-GLOX, designated the K167 vector, were optimized and introduced into rice embryos using Agrobacterium-mediated transformation. Positive transgenic rice embryos were examined using molecular, physiological, biochemical and fermentation tests. The outcomes suggested that SHP degraded lignin effectively.

conclusionsThis research has created a rice breeding material with normal growth and yield but stalks that are more amenable to degradation in the later stage for use in breeding rice varieties whose stalks are easily used for energy. Our results will improve the industrial and commercial applications of rice straw.

Indexed as

Agrobacterium-mediated transformationFermentationglyoxal oxidaseLigninSoybean hull peroxidaseTransgenic rice

Identifiers

PMID35717235
PMCPMC9206748
OpenAlexW4283073987

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.