Evidence map›Paper›PMID 35715714›Full record

ArticleProbiotics and antimicrobial proteins2022

Green Synthesized Silver Nanoparticles Using Lactobacillus Acidophilus as an Antioxidant, Antimicrobial, and Antibiofilm Agent Against Multi-drug Resistant Enteroaggregative Escherichia Coli.

Padikkamannil Abishad, Jess Vergis, Varsha Unni, Vemula Prasastha Ram, Pollumahanti Niveditha, Jyothsana Yasur, Sanis Juliet, Lijo John, Kullaiya Byrappa, Prejit Nambiar and 3 more

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Article in Probiotics and antimicrobial proteins, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Biosynthesis of Silver Nanoparticles viaMolecules (Basel, Switzerland) · 2025
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Padikkamannil AbishadDepartment of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India.
Jess VergisDepartment of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India. itzjessvergis@gmail.com.
Varsha UnniDepartment of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India.
Vemula Prasastha RamICAR- National Research Centre On Meat, Chengicherla, Hyderabad, 500 092, India.
Pollumahanti NivedithaICAR- National Research Centre On Meat, Chengicherla, Hyderabad, 500 092, India.
Jyothsana YasurICAR- National Research Centre On Meat, Chengicherla, Hyderabad, 500 092, India.
Sanis JulietDepartment of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India.
Lijo JohnDepartment of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India.
Kullaiya ByrappaCentre for Research and Innovations, BGSIT, Adichunchanagiri University, B.G. Mandya, 571 418, India.
Prejit NambiarDepartment of Veterinary Public Health, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Lakkidi Post, Wayanad, 673 576, India.
Nitin Vasantrao KurkureDepartment of Veterinary Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur, 440 006, India.
Sukhadeo Baliram BarbuddheICAR- National Research Centre On Meat, Chengicherla, Hyderabad, 500 092, India.
Deepak Bhiwa RawoolICAR- National Research Centre On Meat, Chengicherla, Hyderabad, 500 092, India. deepak.rawool@yahoo.com.ORCID 0000-0002-6173-4708

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study was envisaged to employ the green synthesis and characterization of silver nanoparticles (AgNPs) using the potential probiotic strain Lactobacillus acidophilus, to assess its antibacterial as well as antibiofilm activity against multi-drug-resistant enteroaggregative Escherichia coli (MDR-EAEC) strains and to investigate their antioxidant activity. In this study, AgNPs were successfully synthesized through an eco-friendly protocol, which was then confirmed by its X-ray diffraction (XRD) pattern. A weight loss of 15% up to 182 °C with a narrow exothermic peak between 170 °C and 205 °C was observed in thermogravimetric analysis-differential thermal analysis (TGA-DTA), while aggregated nanoclusters were observed in scanning electron microscopy (SEM). Moreover, the transmission electron microscopy (TEM) imaging of AgNPs revealed a spherical morphology and crystalline nature with an optimum size ranging from 10 to 20 nm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of green synthesized AgNPs against the MDR-EAEC strains were found to be 7.80 mg/L and 15.60 mg/L, respectively. In vitro time-kill kinetic assay revealed a complete elimination of the MDR-EAEC strains after 180 min on co-incubation with the AgNPs. Moreover, the green synthesized AgNPs were found safe by in vitro haemolytic assay. Besides, the green synthesized AgNPs exhibited significant biofilm inhibition (P < 0.001) formed by MDR-EAEC strains. Additionally, a concentration-dependent antioxidant activity was observed in 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. Hence, this study demonstrated potential antibacterial as well as antibiofilm activity of green synthesized AgNPs against MDR-EAEC strains with antioxidant properties and warrants further in-depth studies to explore it as an effective antimicrobial agent against MDR infections.

Indexed as

Anti-Infective AgentsMetal NanoparticlesProbioticsAnti-Bacterial AgentsAntioxidantsBiofilmsEscherichia coliLactobacillus acidophilusMicrobial Sensitivity TestsPlant ExtractsSilverAnti-Bacterial AgentsAnti-Infective AgentsAntioxidantsPlant ExtractsSilverAntimicrobial resistanceEnteroaggregative Escherichia coliGreen synthesisLactobacillus acidophilusNanoparticlesSilver

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.