Evidence map›Paper›PMID 35715603›Full record

ArticleMolecular biology reports2022

Regulation of vtg and VtgR in mud crab Scylla paramamosain by miR-34.

Yinzhen Sheng, Jiaqian Liao, Ziping Zhang, Yuting Li, Xiwei Jia, Xianyuan Zeng, Yilei Wang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. The miRNAsInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yinzhen ShengKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China.
Jiaqian LiaoKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China.
Ziping ZhangCollege of Animal Science, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.
Yuting LiKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China.
Xiwei JiaKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China.
Xianyuan ZengKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China.
Yilei WangKey Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College, Jimei University, 361021, Xiamen, China. ylwang@jmu.edu.cn.ORCID http://orcid.org/0000-0002-9048-1308
Jimei University · CNFujian Agriculture and Forestry University · CN

Funding

Natural Science Foundation of Fujian Province 2019J01691Special Funds Provided by the Ministry of Science and Technology of the People's Republic of China to Guide the Development of Science and Technology in Fujian Province 2020L3011the National Key R&D Program of China 2018YFD0900205the National Natural Science Foundation of China 31672681the National Natural Science Foundation of China 41676161
6 · The paper itself

Abstract

backgroundVitellogenin (Vtg) is the precursor of major yolk protein and plays a crucial role in the maturation of oocytes and the production of eggs in oviparous animals. Vitellogenin receptor (VtgR) mediates the transport of Vtg explicitly to oocytes in the membrane. In a previous study, we found that miR-34 can regulate the expression of some eyestalk genes and affect reproduction in mud crab Scylla paramamosain, one of the most important economic crabs on the coasts of southern China. METHODS AND

resultsIn this study, firstly, we found that miR-34 can target at 3'-UTR of Vtg and VtgR genes by using bioinformatic tools and predicted miR-34 might depress the expression of Vtg and VtgR. Secondly, the relative luciferase activity of HEK293T cells co-transfected with miRNA mimic and pmir-RB-REPORTTM-Vtg/VtgR-3'UTR was significantly lower than those of cells co-transfected with mimic NC and pmir-RB-REPORTTM-Vtg/VtgR-3'UTR. Finally, in vivo experiments showed that agomiR-34 could repress the expression of Vtg and VtgR genes, while Antigomir-34 could promote the expression of these two genes.

conclusionsThese results confirm our hypothesis and previous published results that miR-34 may indirectly regulate ovarian development by binding to the 3'-UTR of Vtg and VtgR genes and inhibiting their expression.

Indexed as

BrachyuraMicroRNAs3' Untranslated RegionsAnimalsEgg ProteinsHEK293 CellsHumansReceptors, Cell SurfaceVitellogenins3' Untranslated RegionsEgg ProteinsMicroRNAsReceptors, Cell Surfacevitellogenin receptorVitellogeninsmiR-34Ovarian developmentScylla paramamosainVtg, VtgR

Identifiers

PMID35715603
OpenAlexW4283032387

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.