ReviewNature protocols2022
Using MetaboAnalyst 5.0 for LC-HRMS spectra processing, multi-omics integration and covariate adjustment of global metabolomics data.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 716 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
716 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Multi-omics time-series analysis in microbiome research: a systematic review.Briefings in bioinformatics · 2025Pooled it
- Oxylipin serum profile changes in response to an open-label anti-inflammatory dietary intervention.Clinical nutrition ESPEN · 2025Trial
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- A bioengineered model of human placental exposure to environmental metals during pregnancy.Nature biomedical engineering · 2026Article
- Host metadherin coordinates hepatic lipid metabolism and CD8Cell metabolism · 2026Article
- A Postbiotic-Inducing Ganoderma applanatum Polysaccharide Repairs the Intestinal Barrier via a Phocaeicola vulgatus - Tridecylic Acid Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Metabolome analyses of the rumen, feces and blood reveal separate and simultaneous impacts of increased dietary concentrate and hindgut starch flow in Holstein cows.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Untargeted plasma metabolomics identifies metabolite signatures associated with suicidality status in chronic schizophrenia.European archives of psychiatry and clinical neuroscience · 2026Article
- Computational metabolomics at scale: from open data to insight.Current opinion in biotechnology · 2026Review
- Untargeted UHPLC-MS/MS analysis of intestines of high-valueFood chemistry: X · 2026Article
- Acute exercise-induced lipolysis and increase in levels of fatty acid esters of hydroxy fatty acids in adipose tissue are impaired in obese and aged mice.Experimental physiology · 2026Article
- Urinary metabolomic signatures of muscle injury and recovery in elite football players.Metabolomics : Official journal of the Metabolomic Society · 2026Observational
- Article
- Integrated Multi-Omics Reveals Complementary Luminal and Mucosal Host-Microbiome Interactions Associated with Disease Activity and Phenotype in Paediatric Inflammatory Bowel Disease.Microorganisms · 2026Article
- MetaboAnnotate: An AI-powered Multiagent Framework for Integrating Annotation Tools for Untargeted Metabolomics.Analytical chemistry · 2026Article
- DietaryAnimals : an open access journal from MDPI · 2026Article
- Assessing the therapeutic potential of PDE-5 inhibitors in adults: Insights from a multi-omics study.Physiological reports · 2026Article
- Tissue-Specific and Spatially Dependent Metabolic Signatures Perturbed by Injury in Male and Female Mice.Arthritis & rheumatology (Hoboken, N.J.) · 2026Article
- Integrated Transcriptomic and Metabolomic Mining of Candidate Genes for Weevil Resistance in Pea.Plants (Basel, Switzerland) · 2026Article
656 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has become a workhorse in global metabolomics studies with growing applications across biomedical and environmental sciences. However, outstanding bioinformatics challenges in terms of data processing, statistical analysis and functional interpretation remain critical barriers to the wider adoption of this technology. To help the user community overcome these barriers, we have made major updates to the well-established MetaboAnalyst platform ( www.metaboanalyst.ca ). This protocol extends the previous 2011 Nature Protocol by providing stepwise instructions on how to use MetaboAnalyst 5.0 to: optimize parameters for LC-HRMS spectra processing; obtain functional insights from peak list data; integrate metabolomics data with transcriptomics data or combine multiple metabolomics datasets; conduct exploratory statistical analysis with complex metadata. Parameter optimization may take ~2 h to complete depending on the server load, and the remaining three stages may be executed in ~60 min.
Indexed as
Identifiers
35715522What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.