ArticleScientific reports2022
Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 24 citations in OpenAlex.
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- Bloodmeal metabarcoding reveals host feeding patterns for Aedes aegypti and Culex quinquefasciatus in Jutiapa, Guatemala and Texas, USA.Scientific reports · 2026Article
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- Bloodmeal metabarcoding reveals host feeding patterns for Aedes aegypti and Culex quinquefasciatus in Jutiapa, Guatemala and Texas, USA.Research square · 2026Article
- Advancing the design of the kissing bug kill trap for surveillance of triatomines.PLoS neglected tropical diseases · 2026Article
- Deforestation effects and house invasion by chagas disease vectors in Brazil.Scientific reports · 2025Article
- Advancing the design of the kissing bug kill trap for surveillance of triatomines.bioRxiv : the preprint server for biology · 2025Article
- Characterizing the transmission dynamics of Trypanosoma cruzi in Triatoma sanguisuga collected from dog kennels in southern Texas.Parasites & vectors · 2025Article
- First whole-genome sequence of Triatoma sanguisuga (Le Conte, 1855), vector of Chagas disease.G3 (Bethesda, Md.) · 2025Article
- Comparative Feeding and Defecation Behaviors ofInsects · 2025Article
- MultiplexPeerJ · 2025Article
- Genome report: First whole genome sequence ofbioRxiv : the preprint server for biology · 2024Article
- Chagas disease in immunocompromised patients.Clinical microbiology reviews · 2024Review
- First Report of Chagas Disease Vector Species Triatoma sanguisuga (Hemiptera: Reduviidae) Infected with Trypanosoma cruzi in Delaware.The American journal of tropical medicine and hygiene · 2024Article
- Canine Systemic Insecticides Fluralaner and Lotilaner Induce Acute Mortality of Triatoma gerstaeckeri, North American Vector of the Chagas Disease Parasite.The American journal of tropical medicine and hygiene · 2023Article
- Microbiomes of Blood-Feeding Triatomines in the Context of Their Predatory Relatives and the Environment.Microbiology spectrum · 2023Article
- Vector mapping and bloodmeal metabarcoding demonstrate risk of urban Chagas disease transmission in Caracas, Venezuela.PLoS neglected tropical diseases · 2023Article
- Effectiveness of fluralaner treatment regimens for the control of canine Chagas disease: A mathematical modeling study.PLoS neglected tropical diseases · 2023Article
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely. We used two methods-Sanger sequencing and amplicon deep sequencing-to target a 228 bp region of the vertebrate Cytochrome b gene and determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct Sanger sequencing of PCR amplicons was successful for 36 samples (31%). Sanger sequencing revealed 15 distinct host species, which included humans, domestic animals (Canis lupus familiaris, Ovis aries, Gallus gallus, Bos taurus, Felis catus, and Capra hircus), wildlife (Rattus rattus, Incilius nebulifer, Sciurus carolinensis, Sciurus niger, and Odocoileus virginianus), and captive animals (Panthera tigris, Colobus spp., and Chelonoidis carbonaria). Samples sequenced by the Sanger method were also subjected to Illumina MiSeq amplicon deep sequencing. The amplicon deep sequencing results (average of 302,080 usable reads per sample) replicated the host community revealed using Sanger sequencing, and detected additional hosts in five triatomines (13.9%), including two additional blood sources (Procyon lotor and Bassariscus astutus). Up to four bloodmeal sources were detected in a single triatomine (I. nebulifer, Homo sapiens, C. lupus familiaris, and S. carolinensis). Enhanced understanding of vector-host-parasite networks may allow for integrated vector management programs focusing on highly-utilized and highly-infected host species.
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