ArticleScientific reports2022
Dual-expression system for blue fluorescent protein optimization.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 33 citations in OpenAlex.
- Actin conformation dynamics precede force generation in cytotoxic T lymphocytes.Communications biology · 2026Article
- Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026Article
- Development of far-red fluorescent proteins for temporal domain multiplexing and super-resolution imaging.Cell reports methods · 2026Article
- WormTagDB: a systematic survey of endogenously tagged proteins in Caenorhabditis elegans and roadmap toward the tagged proteome.G3 (Bethesda, Md.) · 2026Article
- A CandiChrome toolkit for multicolor labeling ofbioRxiv : the preprint server for biology · 2026Article
- A nanobody-based degron system for targeted protein knockdown in Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- WormTagDB: A Systematic Survey of Endogenously Tagged Proteins inbioRxiv : the preprint server for biology · 2025Article
- Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.PLoS biology · 2025Article
- Dopamine signaling drives skin invasion by human-infective nematodes.Nature communications · 2025Article
- The Mla pathway promotesmBio · 2025Article
- Use of blue fluorescent protein Electra2 for live-cell imaging inmicroPublication biology · 2025Article
- Expanding the fluorescent toolkit: Blue fluorescent protein-expressing Plasmodium berghei for enhanced multiplex microscopy.PloS one · 2025Article
- ORP9-PH domain-based fluorescent reporters for visualizing phosphatidylinositol 4-phosphate dynamics in living cells.RSC chemical biology · 2024Article
- Bacillus subtilis: current and future modification strategies as a protein secreting factory.World journal of microbiology & biotechnology · 2024Review
- Light Control in Microbial Systems.International journal of molecular sciences · 2024Review
- A short guide on blue fluorescent proteins: limits and perspectives.Applied microbiology and biotechnology · 2024Review
- YTnC2, an improved genetically encoded green calcium indicator based on toadfish troponin C.FEBS open bio · 2023Article
- Quantitative assessment of near-infrared fluorescent proteins.Nature methods · 2023Article
- Application of Entity-BERT model based on neuroscience and brain-like cognition in electronic medical record entity recognition.Frontiers in neuroscience · 2023Article
- Chaotic medical image encryption method using attention mechanism fusion ResNet model.Frontiers in neuroscience · 2023Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spectrally diverse fluorescent proteins (FPs) provide straightforward means for multiplexed imaging of biological systems. Among FPs fitting standard color channels, blue FPs (BFPs) are characterized by lower brightness compared to other spectral counterparts. Furthermore, available BFPs were not systematically characterized for imaging in cultured mammalian cells and common model organisms. Here we introduce a pair of new BFPs, named Electra1 and Electra2, developed through hierarchical screening in bacterial and mammalian cells using a novel dual-expression vector. We performed systematic benchmarking of Electras against state-of-art BFPs in cultured mammalian cells and demonstrated their utility as fluorescent tags for structural proteins. The Electras variants were validated for multicolor neuroimaging in Caenorhabditis elegans, zebrafish larvae, and mice in comparison with one of the best in the class BFP mTagBFP2 using one-photon and two-photon microscopy. The developed BFPs are suitable for multicolor imaging of cultured cells and model organisms in vivo. We believe that the described dual-expression vector has a great potential to be adopted by protein engineers for directed molecular evolution of FPs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.