Frequency-specific sensitivity of 3T3-L1 preadipocytes to low-intensity vibratory stimulus during adipogenesis.

Oznur Baskan, Oyku Sarigil, Gulistan Mese, Engin Ozcivici

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Article in In vitro cellular & developmental biology. Animal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Oznur BaskanDepartment of Bioengineering, Izmir Institute of Technology, 35430, Urla, Izmir, Turkey.

Oyku SarigilDepartment of Bioengineering, Izmir Institute of Technology, 35430, Urla, Izmir, Turkey.

Gulistan MeseDepartment of Molecular Biology and Genetics, Izmir Institute of Technology, 35430, Urla, Izmir, Turkey.

Engin OzciviciDepartment of Bioengineering, Izmir Institute of Technology, 35430, Urla, Izmir, Turkey. enginozcivici@iyte.edu.tr.ORCID http://orcid.org/0000-0003-4464-0475

Funding

Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 111M604Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 111T577Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 215S862Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 2211-A

6 · The paper itself

Abstract

Adipocyte accumulation in the bone marrow is a severe complication leading to bone defects and reduced regenerative capacity. Application of external mechanical signals to bone marrow cellular niche is a non-invasive and non-pharmaceutical methodology to improve osteogenesis and suppress adipogenesis. However, in the literature, the specific parameters related to the nature of low-intensity vibratory (LIV) signals appear to be arbitrarily selected for amplitude, bouts, and applied frequency. In this study, we performed a LIV frequency sweep ranging from 30 to 120 Hz with increments of 15 Hz applied onto preadipocytes during adipogenesis for 10 d. We addressed the effect of LIV with different frequencies on single-cell density, adipogenic gene expression, lipid morphology, and triglycerides content. Results showed that LIV signals with 75-Hz frequency had the most significant suppressive effect during adipogenesis. Our results support the premise that mechanical-based interventions for suppressing adipogenesis may benefit from optimizing input parameters.

Indexed as

AdipocytesAdipogenesis3T3-L1 CellsAnimalsBone Marrow CellsCell DifferentiationMiceOsteogenesisAdipogenic differentiationBone marrowFrequencyLow-intensity vibrationsMagnetic levitationPreadipocytesSingle-cell density

Identifiers

PMID35713773

DOI10.1007/s11626-022-00696-5

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.