ArticleMagnetic resonance in medicine2022
Quantitative cerebrovascular reactivity MRI in mice using acetazolamide challenge.
Article in Magnetic resonance in medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Acetazolamide inhibition of carbonic anhydrase 4 reverses opioid-induced synaptic rearrangements in nucleus accumbens and reduces drug-seeking behavior.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- Vascular smooth muscle cell loss, but not neuroinflammation, drives cerebrovascular reactivity impairment in Alzheimer's disease.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Article
- Bidirectional crusher gradient method to estimate the labeling efficiency of pseudo-continuous arterial spin labeling MRI in mice.Magnetic resonance in medicine · 2026Article
- Vascular smooth muscle cell loss, but not neuroinflammation, drives cerebrovascular reactivity impairment in Alzheimer's disease.bioRxiv : the preprint server for biology · 2025Article
- Magnetic resonance imaging cerebral blood perfusion measurement in awake mice.Journal of neuroscience methods · 2025Article
- Carbonic anhydrase 4 disruption and pharmacological inhibition reduce synaptic and behavioral adaptations following oxycodone withdrawal.bioRxiv : the preprint server for biology · 2025Article
- Vascular and metabolic responses to elevated circulating PDGF-BB in mice: A multiparametric MRI study.Health and metabolism · 2025Article
- The impact of isoflurane anesthesia on brain metabolism in mice: An MRI and electroencephalography study.NMR in biomedicine · 2024Article
- Article
- Bone-derived PDGF-BB drives brain vascular calcification in male mice.The Journal of clinical investigation · 2023Article
- Non-contrast assessment of blood-brain barrier permeability to water in mice: An arterial spin labeling study at cerebral veins.NeuroImage · 2023Article
- Toward accurate cerebral blood flow estimation in mice after accounting for anesthesia.Frontiers in physiology · 2023Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo develop a quantitative MRI method to estimate cerebrovascular reactivity (CVR) in mice.
methodsWe described an MRI procedure to measure cerebral vasodilatory response to acetazolamide (ACZ), a vasoactive agent previously used in human clinical imaging. Vascular response was determined by cerebral blood flow (CBF) measured with phase-contrast or pseudo-continuous arterial spin labeling MRI. Vasodilatory input intensity was determined by plasma ACZ level using high-performance liquid chromatography. We verified the source of the CVR MRI signal by comparing ACZ injection to phosphate-buffered saline injection and noninjection experiments. Dose dependence and feasibility of regional CVR measurement were also investigated.
resultsCerebral blood flow revealed an exponential increase following intravenous ACZ injection, with a time constant of 1.62 min. In contrast, phosphate-buffered saline or noninjection exhibited a slow linear CBF increase, consistent with a gradual accumulation of anesthetic agent, isoflurane, used in this study. When comparing different ACZ doses, injections of 30, 60, 120, and 180 mg/kg yielded a linear increase in plasma ACZ concentration (p < 0.0001). On the other hand, CBF changes under these doses were not different from each other (p = 0.50). The pseudo-continuous arterial spin labeling MRI with multiple postlabeling delays revealed similar vascular responses at different postlabeling delay values. There was a regional difference in CVR (p = 0.005), with isocortex (0.81 ± 0.17%/[μg/ml]) showing higher CVR than deep-brain regions. Mice receiving multiple ACZ injections lived for a minimum of 6 months after the study without noticeable aberrant behavior or appearance.
conclusionsWe demonstrated the proof-of-principle of a new quantitative CVR mapping technique in mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.