A simple colorimetric assay to determine the concentration and proportion of human mercaptalbumin.

Sawaki Tada, Keiko Yasukawa, Yutaka Yatomi, Tomoaki Uchiki

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Article in Practical laboratory medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

1.9field-weighted citation impact, top 14% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin.Antioxidants (Basel, Switzerland) · 2022
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sawaki TadaR&D Headquarters, Nitto Boseki Co. Ltd., Kawasaki, Kanagawa, 210-0821, Japan.

Keiko YasukawaDepartment of Clinical Laboratory, The University of Tokyo Hospital, Bunkyo-ku, Tokyo, 113-8655, Japan.

Yutaka YatomiDepartment of Clinical Laboratory, The University of Tokyo Hospital, Bunkyo-ku, Tokyo, 113-8655, Japan.

Tomoaki UchikiR&D Headquarters, Nitto Boseki Co. Ltd., Kawasaki, Kanagawa, 210-0821, Japan.

Nitto Boseki (Japan) · JPUniversity of Tokyo Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: Human serum albumin can take on two forms, mercaptalbumin (HMA) or non-mercaptalbumin (HNA), depending on the redox status of its Cys34. The ratio of HMA and HNA is considered to be a novel biomarker of oxidative stress. While HPLC and mass spectrometry are established methods to measure HMA and HNA, a simple colorimetric assay was applied to measure this biomarker. Design and methods: Michler's Hydrol (4,4'-Bis(dimethylamino)benzhydrol) is a blue dye with a maximum absorption at 612 nm, and its absorption decreases when it reacts with a thiol group. Concentrations of HMA in serum samples from 36 healthy subjects were measured based on absorption changes of Michler's Hydrol. The proportion of HMA (HMA%) in total albumin was also obtained by dividing the HMA concentration by total albumin concentration, which was obtained by a bromocresol purple (BCP) assay. The proportion of HNA (HNA%) was obtained by subtracting HMA% from 100%. Results: HMA concentrations obtained by Michler's Hydrol assay were highly correlated (r Conclusions: A colorimetric assay using Michler's Hydrol was optimized for a 96-well plate format so that it can be easily performed in a standard laboratory setting. This assay gives HMA concentrations and HNA proportions comparable to HPLC.

Indexed as

4,4′-bis(dimethylamino)benzhydrolA612, Absorbance at 612 nmAlbumin redox statesBCP, Bromocresol purpleCKD, Chronic kidney diseaseCys34Cysteine 34HMAHMA, Human mercaptalbuminHNAHNA, Human non-mercaptalbuminHSA, Human Serum AlbuminHuman mercaptalbuminHuman non-mercaptalbuminHuman serum albuminMichler's hydrolOxidative stressOxidized albuminReduced albumin

Identifiers

PMID35711386

DOI10.1016/j.plabm.2022.e00281

PMCPMC9192801

OpenAlexW4281295556

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LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.