Evidence map›Paper›PMID 35711152›Full record

ArticleMicroscopy (Oxford, England)2022

Real-time scanning electron microscopy of unfixed tissue in the solution using a deformable and electron-transmissive film.

Seine A Shintani, Seiji Yamaguchi, Hiroaki Takadama

Open access · hybridAbstract read
In one paragraph

Article in Microscopy (Oxford, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 65% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Seine A ShintaniDepartment of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan.ORCID 0000-0002-1084-2549
Seiji YamaguchiDepartment of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan.
Hiroaki TakadamaDepartment of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan.
Chubu University · JP

Funding

Chubu University 21M01CPChubu University Project (6)Japan Society for the Promotion of Science JP17K15102Japan Society for the Promotion of Science JP20K15762Ministry of Internal Affairs and Communications SCOPENew Energy and Industrial Technology Development Organization JPNP20004
6 · The paper itself

Abstract

It is difficult to use scanning electron microscopy to observe the structure and movement of biological tissue immersed in the solution. To enable such observations, we created a highly deformable and electron-transmissive polyimide film that can withstand the pressure difference between the high-vacuum electron column and the atmospheric-pressure sample chamber. With this film, we used scanning electron microscopy to measure the intrinsic fine structure and movement of the contractile fibers of excised mouse heart immersed in physiological solutions. Our measurements revealed that the excised heart is a dynamic tissue that undergoes relaxation oscillation based on a three-dimensional force balance.

Indexed as

ElectronsAnimalsMiceMicroscopy, Electron, ScanningVacuumdeformable and electron-transmissive filmelectron microscopy of submerged samplesmechanical heart oscillationreal-time electron microscopysarcomeric oscillationsscanning electron microscope

Identifiers

PMID35711152
PMCPMC9535786
OpenAlexW4283071274

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.