ArticleNature communications2022
Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37citing papers in PubMed
5.2field-weighted citation impact, top 3% of its field
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Who cites it
37 citing papers in PubMed, 53 citations in OpenAlex.
- Malaria serology for surveillance, insights into immunity, and vaccine development.Infection and immunity · 2026Review
- The Role of CD4 T Cell Repertoire and Immune Memory Mechanisms in Vaccination and Infection Immunity.Immunological reviews · 2026Review
- Human primary antibody response to vaccination follows a partially sequential class-switching program with a checkpoint at IGHG2.Cell reports. Medicine · 2026Article
- Critical illness expands a transcriptionally distinct hypometabolic CD8bioRxiv : the preprint server for biology · 2026Article
- The buried S2 apex of SARS-CoV-2 spike elicits an immunodominant germline-restricted public antibody response.bioRxiv : the preprint server for biology · 2026Article
- Article
- The Application of Single-Cell Technologies for Vaccine Development Against Viral Infections.Vaccines · 2025Review
- A Versatile High-Throughput Single-Cell Screening Platform for Profiling Antigen-Specific Long-Lived B Cells in Blood and Bone Marrow.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- PTEN inhibits scavenger receptor-mediated phagocytosis of methicillin-resistant Staphylococcus aureus.ImmunoHorizons · 2025Article
- Single-Cell RNA Sequencing of Baseline Immune Profiles After Third Vaccination Associated with Subsequent SARS-CoV-2 Infection in Naïve Individuals.International journal of molecular sciences · 2025Article
- Evaluation of the efficacy of the SARS-CoV-2 vaccine additional and booster doses in immunocompromised patients with multiple sclerosis: the COVACiMS study.Journal of neurology · 2025Article
- Single-cell transcriptome profile during the antibody decline phase following inactivated COVID-19 vaccination.Frontiers in cellular and infection microbiology · 2025Article
- Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires.Communications biology · 2024Article
- Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro.Communications biology · 2024Article
- sciCSR infers B cell state transition and predicts class-switch recombination dynamics using single-cell transcriptomic data.Nature methods · 2024Article
- Single-cell RNA sequencing to understand host-virus interactions.Virologica Sinica · 2024Review
- SingPro: a knowledge base providing single-cell proteomic data.Nucleic acids research · 2024Article
- Negative binomial mixture model for identification of noise in antibody-antigen specificity predictions from single-cell data.Bioinformatics advances · 2024Article
- A multicenter phase Ib trial of the histone deacetylase inhibitor entinostat in combination with pembrolizumab in patients with myelodysplastic syndromes/neoplasms or acute myeloid leukemia refractory to hypomethylating agents.Annals of hematology · 2024Article
- Evaluation of inflammatory biomarkers and their association with anti-SARS-CoV-2 antibody titers in healthcare workers vaccinated with BNT162B2.Frontiers in immunology · 2024Article
Corrections and comments
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Authors and funding
20 authors at 3 institutions in 2 countries.
Kevin J Kramer *Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Erin M Wilfong *Department of Medicine, Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.ORCID 0000-0002-6584-1578
Kelsey Voss *Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.ORCID 0000-0003-1732-2682
Sierra M BaroneDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.ORCID 0000-0001-5944-750X
Andrea R ShiakolasDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Nagarajan RajuDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Caroline E RoeDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Naveenchandra SuryadevaraVanderbilt Vaccine Center, Nashville, TN, 37232, USA.ORCID 0000-0003-2571-1290
Lauren M WalkerDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Steven C WallDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.ORCID 0000-0002-1220-7020
Ariana PauloDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
Samuel SchaeferHuman Immunology Discovery Initiative of the Vanderbilt Center for Immunobiology, Nashville, TN, 37232, USA.ORCID 0000-0002-8175-1791
Debolanle DahunsiDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.ORCID 0000-0002-5230-022X
Camille S WestlakeDepartment of Medicine, Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.
James E CroweVanderbilt Vaccine Center, Nashville, TN, 37232, USA.ORCID 0000-0002-0049-1079
Robert H CarnahanVanderbilt Vaccine Center, Nashville, TN, 37232, USA.ORCID 0000-0001-5230-1532
Jeffrey C RathmellDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. jeff.rathmell@vumc.org.ORCID 0000-0002-4106-3396
Rachel H BonamiDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. rachel.h.bonami@vumc.org.ORCID 0000-0001-7575-6943
Ivelin S GeorgievDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. ivelin.georgiev@vumc.org.ORCID 0000-0002-6312-7696
Jonathan M IrishDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA. jonathan.irish@vanderbilt.edu.ORCID 0000-0001-9428-8866
Vanderbilt Health · USVanderbilt University · USVanderbilt University Medical Center · US
Funding
Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI STACIE B DUSETZINA · 1995 to 2026
$172.8MThe Vanderbilt Institute for Clinical and Translational Research (VICTR)UL1TR000445 · NCATS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BERNARD, GORDON RAPHAEL · 2012 to 2016
$41.4MBuilding Interdisciplinary Research Careers in Women's HealthK12HD043483 · NICHD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI HARTMANN, KATHERINE E, MAJOR, AMY S · 2002 to 2023
$10.3MB-CELL EPITOPE DISCOVERY AND MECHANISMS OF ANTIBODY PROTECTION: Modification for SARS-CoV2 Research75N93019C00074 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI CROWE, JAMES E · 2019 to 2024
$10.2MInstitutional Career Development CoreKL2TR002245 · NCATS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Julie A. Bastarache · 2017 to 2026
$9.3MSingle-Cell Biology and Data Analysis Shared Resource (SCB-DA SR)U54CA217450 · NCI · VANDERBILT UNIVERSITY · PI QUARANTA, VITO · 2018 to 2022
$8.5MMOLECULAR BIOPHYSICS TRAINING PROGRAM AT VANDERBILTT32GM008320 · NIGMS · VANDERBILT UNIVERSITY · PI CHAZIN, WALTER J. · 1989 to 2023
$7.9MSingle Cell Methods for Bioeffector Discovery and AnalysisR01CA226833 · NCI · VANDERBILT UNIVERSITY · PI BRIAN O BACHMANN, Jonathan Michael Irish · 2019 to 2026
$6.1MHuman antibody-based countermeasures against the Wuhan Coronavirus SARS-CoV-2R01AI157155 · NIAID · WASHINGTON UNIVERSITY · PI BARIC, RALPH S, CROWE, JAMES E · 2020 to 2024
$6.0MExploiting metabolic vulnerabilities of CD4 T cell subsets to control inflammatory diseaseR01DK105550 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Jeffrey C Rathmell · 2015 to 2026
$4.7MNeutralization Fingerprinting Analysis of Polyclonal Antibody Responses against HIV-1R01AI131722 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI GEORGIEV, IVELIN · 2017 to 2021
$4.5MMetabolic Barriers to T Cell Activation in Clear Cell Renal Cell CarcinomaR01CA217987 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Jeffrey C Rathmell · 2018 to 2026
$4.3MNCATS NIH HHS KL2 TR002245NCATS NIH HHS UL1 TR000445NCI NIH HHS P30 CA068485NCI NIH HHS R01 CA217987NCI NIH HHS R01 CA226833NCI NIH HHS U54 CA217450NIAID NIH HHS 75N93019C00074NIAID NIH HHS R01 AI131722NIAID NIH HHS R01 AI153167NIAID NIH HHS R01 AI157155NIAID NIH HHS U01 AI125056NICHD NIH HHS K12 HD043483NIDDK NIH HHS R01 DK105550NIDDK NIH HHS T32 DK101003NIGMS NIH HHS T32 GM008320
Abstract
RNA-based vaccines against SARS-CoV-2 have proven critical to limiting COVID-19 disease severity and spread. Cellular mechanisms driving antigen-specific responses to these vaccines, however, remain uncertain. Here we identify and characterize antigen-specific cells and antibody responses to the RNA vaccine BNT162b2 using multiple single-cell technologies for in depth analysis of longitudinal samples from a cohort of healthy participants. Mass cytometry and unbiased machine learning pinpoint an expanding, population of antigen-specific memory CD4
Indexed as
COVID-19COVID-19 VaccinesAntibodies, ViralBNT162 VaccineCD8-Positive T-LymphocytesHumansImmunoglobulin GmRNA VaccinesProteomicsRNA, ViralSARS-CoV-2Vaccines, SyntheticAntibodies, ViralBNT162 VaccineCOVID-19 VaccinesImmunoglobulin GmRNA VaccinesRNA, ViralVaccines, Synthetic
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