Reference-aided full-length transcript assembly, cDNA cloning, and molecular characterization of coronatine-insensitive 1b (COI1b) gene in coconut (Cocos nucifera L.).

Frenzee Kroeizha L Pammit, Anand Noel C Manohar, Darlon V Lantican, Jen Daine L Nocum, Roanne R Gardoce, Hayde F Galvez

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Frenzee Kroeizha L PammitGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines. flpammit@alum.up.edu.ph.ORCID http://orcid.org/0000-0001-9720-519X

Anand Noel C ManoharGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines.ORCID http://orcid.org/0000-0003-2500-1539

Darlon V LanticanGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines.ORCID http://orcid.org/0000-0002-1626-4049

Jen Daine L NocumGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines.ORCID http://orcid.org/0000-0002-7747-8112

Roanne R GardoceGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines.ORCID http://orcid.org/0000-0001-8232-7661

Hayde F GalvezGenetics Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Laguna, Philippines.

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6 · The paper itself

Abstract

backgroundIn the Philippines, 26% of the total agricultural land is devoted to coconut production making coconut one of the most valuable industrial crop in the country. However, the country's multimillion-dollar coconut industry is threatened by the outbreak of coconut scale insect (CSI) and other re-emerging insect pests promoting national research institutes to work jointly on developing new tolerant coconut varieties. Here, we report the cloning and characterization of coronatine-insensitive 1 (COI1) gene, one of the candidate insect defense genes, using 'Catigan Green Dwarf' (CATD) genome sequence assembly as reference. METHODS AND

resultsTwo (2) splicing variants were identified and annotated-CnCOI1b-1 and CnCOI1b-2. The full-length cDNA of CnCOI1b-1 was 7919 bp with an ORF of 1176 bp encoding for a deduced protein of 391 amino acids while CnCOI1b-2 has 2360 bp full-length cDNA with an ORF of 1743 bp encoding a deduced protein of 580 amino acids. The 3D structural model for the two (2) isoforms were generated through homology modelling. Functional analysis revealed that both isoforms are involved in various physiological and developmental plant processes including defense response of plants to insects and pathogens. Phylogenetic analysis confirms high degree of COI1 protein conservation during evolution, especially among monocot species. Differential gene expression via qRT-PCR analysis revealed a seven-fold increase of COI1 gene expression in coconut post introduction of CSI relative to base levels.

conclusionThis study provided the groundwork for further research on the actual role of COI1 in coconut in response to insect damage. The findings of this study are also vital to facilitate the development of improved insect-resistant coconut varieties for vibrant coconut industry.

Indexed as

Amino AcidsCocosCloning, MolecularDNA, ComplementaryIndenesPhylogenyAmino AcidscoronatineDNA, ComplementaryIndenesCoconutCoconut scale insectCoronatine-insensitive 1 geneFull-length transcriptGene expressionGenomics

Identifiers

PMID35708860

DOI10.1007/s11033-022-07658-w

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