Evidence map›Paper›PMID 35708765›Full record

ArticleArchives of virology2022

Genome sequence and experimental infection of calves with bovine gammaherpesvirus 4 (BoHV-4).

Fernando V Bauermann, Shollie M Falkenberg, Mathias Martins, Rohana P Dassanayake, John D Neill, Julia F Ridpath, Simone Silveira, Mitchel V Palmer, Alaine Buysse, Anna Mohr and 2 more

Abstract read
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Article in Archives of virology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Fernando V Bauermann *Department of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175 North Campus Drive, Brookings, SD, 57007, USA.
Shollie M Falkenberg *Ruminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
Mathias Martins *Department of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175 North Campus Drive, Brookings, SD, 57007, USA.
Rohana P DassanayakeRuminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
John D NeillRuminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
Julia F RidpathRuminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
Simone SilveiraRuminant Disease and Immunology Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
Mitchel V PalmerInfectious Bacterial Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 1920 Dayton Avenue, P.O. Box 70, Ames, IA, 50010, USA.
Alaine BuysseDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175 North Campus Drive, Brookings, SD, 57007, USA.
Anna MohrDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175 North Campus Drive, Brookings, SD, 57007, USA.
Eduardo F FloresSetor de Virologia, Universidade Federal de Santa Maria, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil.
Diego G DielDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175 North Campus Drive, Brookings, SD, 57007, USA. dgdiel@cornell.edu.ORCID http://orcid.org/0000-0003-3237-8940
Agricultural Research Service · USSouth Dakota State University · USOklahoma State University · USUniversidade Federal de Santa Maria · BR

Funding

National Institute of Food and Agriculture NYC-478419National Institute of Food and Agriculture SD00H517-14
6 · The paper itself

Abstract

Bovine gammaherpesvirus 4 (BoHV-4) is ubiquitous in cattle worldwide, and it has been detected in animals exhibiting broad clinical presentations. The virus has been detected in the United States since the 1970s; however, its clinical relevance remains unknown. Here, we determined the complete genome sequences of two contemporary BoHV-4 isolates obtained from respiratory (SD16-38) or reproductive (SD16-49) tract specimens and assessed clinical, virological, and pathological outcomes upon intranasal (IN) inoculation of calves with the respiratory BoHV-4 isolate SD16-38. A slight and transient increase in body temperature was observed in BoHV-4-inoculated calves. Additionally, transient viremia and virus shedding in nasal secretions were observed in all inoculated calves. BoHV-4 DNA was detected by nested PCR in the tonsil and regional lymph nodes (LNs) of calves euthanized on day 5 post-inoculation (pi) and in the lungs of calves euthanized on day 10 pi. Calves euthanized on day 35 pi harbored BoHV-4 DNA in the respiratory tract (turbinates, trachea, lungs), regional lymphoid tissues, and trigeminal ganglia. Interestingly, in situ hybridization revealed the presence of BoHV-4 DNA in nerve bundles surrounding the trigeminal ganglia and retropharyngeal lymph nodes (day 35 pi). No histological changes were observed in the respiratory tract (turbinate, trachea, and lung), lymphoid tissues (tonsil, LNs, thymus, and spleen), or central nervous tissues (olfactory bulb and trigeminal ganglia) sampled throughout the animal studies (days 5, 10, and 35 pi). This study contributes to the understanding of the infection dynamics and tissue distribution of BoHV-4 following IN infection in calves. These results suggest that BoHV-4 SD16-38 used in our study has low pathogenicity in calves upon intranasal inoculation.

Indexed as

Cattle DiseasesHerpesviridae InfectionsHerpesvirus 1, BovineHerpesvirus 4, BovineAnimalsAntibodies, ViralCattleVirus SheddingAntibodies, Viral

Identifiers

PMID35708765
OpenAlexW4283011998

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.