ArticleEuropean journal of nutrition2022
Ameliorating high-fat diet-induced sperm and testicular oxidative damage by micronutrient-based antioxidant intervention in rats.
Article in European journal of nutrition, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Kisspeptin-10 Alleviates Obesity-induced Testicular Oxidative Stress and Mitochondrial Dysfunction via Modulation of Mitophagy and Autophagy Signaling.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Timing-Dependent Effects of Prebiotic-Probiotic Supplementation on High-Fat-Diet-Induced Testicular Dysfunction and Gut Microbiota Alterations in Rats.Microorganisms · 2026Article
- Ameliorative Effect of Policosanol on High-Fat Diet-Induced Testicular Dysfunction: Implications of ROS/SIRT1 and Apoptotic Signaling Pathways in Rats.Chemistry & biodiversity · 2026Article
- Clove-Chitosan Nanoparticles Alleviate Fertility Impairment and Reproductive Dysfunction in Obese Male Rat Model.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Probiotics as Microbiome Modulators in Male Infertility: Rethinking Dysbiosis Across the Gut-Testis Axis.Journal of personalized medicine · 2026Review
- Obesity in reproduction: Mechanisms from fertilization to post‑uterine development (Review).International journal of molecular medicine · 2025Review
- Modified Liuwei Dihuang Decoction Ameliorates Oligoasthenozoospermia in Mice via Modulation of the PI3K/AKT/Nrf2 Signaling Pathway.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Effect of High-Intensity Interval Training on Mitigating High-Fat Diet-Induced Damage in Rat Testicular Tissue.Preventive nutrition and food science · 2025Article
- Enhancing salinity tolerance in cultivated rice through introgression of African rice genes and application of moringa leaf extract.BMC plant biology · 2025Article
- Blood plasma trimethylamine N-oxide and related metabolites and asthenozoospermia odds: a hospital-based matched case-control study in China.Human reproduction open · 2025Article
- Effect of chitosan on buck semen quality and semen plasma metabolites during low-temperature storage.Frontiers in veterinary science · 2025Article
- Integrating proteomics and metabolomics to evaluate impact of semen collection techniques on the quality and cryotolerance of goat semen.Scientific reports · 2024Article
- Antioxidants in Male and Animal Reproduction: Applications and Critical Issues.Antioxidants (Basel, Switzerland) · 2024Article
- Reliable Detection of Excessive Sperm Ros Production in Subfertile Patients: How Many Men with Oxidative Stress?Antioxidants (Basel, Switzerland) · 2024Article
- Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles.International journal of molecular sciences · 2024Article
- Effect of Advanced Glycation end Products (AGEs) on Sperm Parameters and Function in C57Bl/6 Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Preconceptional paternal caloric restriction of high-fat diet-induced obesity in Wistar rats dysregulates the metabolism of their offspring via AMPK/SIRT1 pathway.Lipids in health and disease · 2024Article
- Article
- Review
- A short-term high-fat diet alters rat testicular activity and blood-testis barrier integrity through the SIRT1/NRF2/MAPKs signaling pathways.Frontiers in endocrinology · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeEmerging evidence from rodent studies suggests that high-fat-diet (HFD)-induced obesity is characterized by increased oxidative damage in sperm and testis. However, interventions using micronutrient supplementation to mitigate oxidative damage in obesity have not been extensively studied. This study aimed to investigate the effect of an antioxidant-based micronutrient supplement (added folate, vitamin B
methodsRats (3-weeks-old, 12/group) were weaned onto control (C) or HFD (H) or these diets with micronutrient supplement (CS; HS); sperm and testis were harvested at 30.5 weeks. To assess oxidative stress and antioxidant capacity in testis, levels of malondialdehyde (MDA), glutathione (GSH), folate and susceptibility index (SI) of pro-oxidative damage, mRNA expression of Nrf2, NFκB-p65, IL-6, IL-10 and TNF-α, in addition to superoxide-dismutase (SOD), catalase and glutathione-peroxidase (GPx) activities were measured. 8-hydroxy-2-deoxyguanosine (8-OHdG) were assessed in both sperm and testis.
resultsHFD-fed rats had significantly increased 8-OHdG content in sperm and testis, increased testicular SI, decreased testicular weight, SOD and GPx activity compared to control. Strikingly, supplementation of HFD appeared to significantly reduce 8-OHdG in sperm and testis (22% and 24.3%, respectively), reduce testicular SI and MDA content (28% and 40%, respectively), increase testicular weight (24%), SOD and GPX activity (30% and 70%, respectively) and GSH content (19%). Moreover, supplementation had significant impact to increase testicular folate content regardless of diet. Furthermore, an overall effect of supplementation to increase testicular mRNA expression of Nrf2 was observed across groups. Interestingly, testicular SI was positively correlated with sperm and testicular 8-OHdG and MDA content, suggesting a critical role of testicular antioxidant activity to combat oxidative damage in sperm and testis.
conclusionOur findings suggest that antioxidant-based micronutrient supplement has the potential to interrupt HFD-induced sperm and testicular oxidative damage by improving testicular antioxidant capacity.
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