ArticleApplied microbiology and biotechnology2022
One-pot platform for rapid detecting virus utilizing recombinase polymerase amplification and CRISPR/Cas12a.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- A field deployable duplex RPA-CRISPR/Cas12a assay for rapid and sensitive detection of African swine fever virus.Archives of virology · 2026Article
- A Microfluidic Chip and a Portable Colorimetric Detection Device for the Rapid, Low-Cost, and Accurate Diagnosis of ASFV.Transboundary and emerging diseases · 2026Article
- Development of rapid multiplex human herpesvirus detection systems based on recombinase polymerase amplification and a lateral flow assay.Frontiers in cell and developmental biology · 2026Article
- Enhanced One-Pot Cas12a-Based Nucleic Acid Detection via Epitope Insertion and Recruitment of Rad51.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- The application of the CRISPR-Cas system in Klebsiella pneumoniae infections.Molecular biology reports · 2025Review
- CRISPR/Cas12a-Based One-Tube RT-RAA Assay for PoRV Genotyping.International journal of molecular sciences · 2025Article
- The establishment and optimization of aMicrobiology spectrum · 2025Article
- Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay.Frontiers in microbiology · 2025Article
- Rapid and equipment-free identification of papaya mealybug Paracoccus marginatus based on RPA-CRISPR/Cas12a.Pest management science · 2025Article
- Sensitive, Semiquantitative, and Portable Nucleic Acid Detection of Rabies Virus Using a Personal Glucose Meter.ACS omega · 2024Article
- CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus.ACS sensors · 2024Article
- Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy.RSC advances · 2024Article
- Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay.BMC veterinary research · 2024Article
- CRISPR-based diagnostics detects invasive insect pests.Molecular ecology resources · 2024Article
- Rapid detection ofFrontiers in microbiology · 2024Article
- A rapid and ultra-sensitive dual readout platform forFrontiers in cellular and infection microbiology · 2024Article
- Establishment of a platform based on dual RPA combined with CRISPR/Cas12a for the detection ofFrontiers in bioengineering and biotechnology · 2024Article
- CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.Biosensors · 2023Article
- Rapid Visual Detection of African Swine Fever Virus with a CRISPR/Cas12a Lateral Flow Strip Based on Structural Protein Gene D117L.Animals : an open access journal from MDPI · 2023Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The livestock industry has been deeply affected by African swine fever virus (ASFV) and Capripoxvirus (CaPV), which caused an enormous economic damage. It is emergent to develop a reliable detection method. Here, we developed a rapid, ultra-sensitive, and one-pot DNA detection method combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a for ASFV and CaPV, named one-pot-RPA-Cas12a (OpRCas) platform. It had the virtue of both RPA and CRISPR/Cas12a, such as high amplification efficiency, constant temperature reaction, and strict target selectivity, which made diagnosis simplified, accurate and easy to be operated without expensive equipment. Meanwhile, the reagents of RPA and CRISPR/Cas12a were added to the lid and bottom of tube in one go, which overcame the incompatibility of two reactions and aerosol contamination. To save cost, we only need a quarter of the amount of regular RPA per reaction which is enough to achieve clinical diagnosis. The OpRCas platform was 10 to 100 times more sensitive than qPCR; the limit of detection (LOD) was as low as 1.2 × 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.