ArticleJournal of tissue engineering
A gastruloid model of the interaction between embryonic and extra-embryonic cell types.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.FEBS letters · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Modeling Post-Implantation Mammalian Embryogenesis Using AdvancedInternational journal of molecular sciences · 2026Review
- Dual-patterned pluripotent stem cells self-organize into a human embryo model with extended anterior-posterior patterning.bioRxiv : the preprint server for biology · 2025Article
- Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells.FEBS open bio · 2025Article
- Advances in engineered models of peri-gastrulation.iScience · 2025Review
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- Topical section: embryonic models (2023) for Current Opinion in Genetics & Development.Current opinion in genetics & development · 2024Review
- The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion.PLoS computational biology · 2024Article
- Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Constructing the pharyngula: Connecting the primary axial tissues of the head with the posterior axial tissues of the tail.Cells & development · 2023Review
- Wnt and BMP signalling direct anterior-posterior differentiation in aggregates of mouse embryonic stem cells.Biology open · 2023Article
- Multimodal characterization of murine gastruloid development.Cell stem cell · 2023Article
- Stem cell-based models of early mammalian development.Development (Cambridge, England) · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem-cell derived in vitro systems, such as organoids or embryoids, hold great potential for modeling in vivo development. Full control over their initial composition, scalability, and easily measurable dynamics make those systems useful for studying specific developmental processes in isolation. Here we report the formation of gastruloids consisting of mouse embryonic stem cells (mESCs) and extraembryonic endoderm (XEN) cells. These XEN-enhanced gastruloids (XEGs) exhibit the formation of neural epithelia, which are absent in gastruloids derived from mESCs only. By single-cell RNA-seq, imaging, and differentiation experiments, we demonstrate the neural characteristics of the epithelial tissue. We further show that the mESCs induce the differentiation of the XEN cells to a visceral endoderm-like state. Finally, we demonstrate that local inhibition of WNT signaling and production of a basement membrane by the XEN cells underlie the formation of the neuroepithelial tissue. In summary, we establish XEGs to explore heterotypic cellular interactions and their developmental consequences in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.