ArticleThe New phytologist2022
Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- The two-component regulator CvsR has a small core regulonMicrobiology spectrum · 2026Article
- Unlocking the genetic arsenal of Xanthomonas arboricola: new insights into taxonomic classification, pathogenicity and adaptation beyond the effectorome.BMC genomics · 2026Article
- A Genome-Wide Mutant Screen Identifies XopN and XopX as Core Type III Effectors Required for Peach Infection byMicroorganisms · 2026Article
- Lactuchelins represent lipopeptide siderophores produced by Pseudomonas lactucae that inhibit Xanthomonas campestris.The ISME journal · 2026Article
- Unraveling fitness landscapes in plant-associated Bacteroidota.ISME communications · 2026Article
- The two-component regulator CvsR has a small core regulonbioRxiv : the preprint server for biology · 2025Article
- Bacterial fitness for plant colonization is influenced by plant growth substrate.The New phytologist · 2025Article
- Construction of a randomly barcoded insertional mutant library in the filamentous fungusbioRxiv : the preprint server for biology · 2025Article
- Comparative transcriptomics reveals a highly polymorphic Xanthomonas HrpG virulence regulon.BMC genomics · 2024Article
- Review
- Chromosomal barcodes for simultaneous tracking of near-isogenic bacterial strains in plant microbiota.Nature microbiology · 2024Article
- Bacterial host adaptation through sequence and structural variations of a single type III effector gene.iScience · 2024Article
- A large scale bacterial attraction assay: A new quantitative bacterial migration assay suitable for genetic screens.PloS one · 2024Article
- Integration of mRNA and miRNA analysis reveals the molecular mechanisms of sugar beet (Beta vulgaris L.) response to salt stress.Scientific reports · 2023Article
- Dissimilar gene repertoires ofFrontiers in plant science · 2023Article
- A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differencesin Fitness during Plant Infection.Bio-protocol · 2022Article
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
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