Evidence map›Paper›PMID 35706372›Full record

ArticleYeast (Chichester, England)2022

A method for quantifying sporulation efficiency and isolating meiotic progeny in non-GMO strains of Saccharomyces cerevisiae.

Amy Sirr, Martin S Timour, Gareth A Cromie, Michelle Tang, Aimée M Dudley

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Yeast (Chichester, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Amy SirrPacific Northwest Research Institute, Seattle, Washington, USA.
Martin S TimourPacific Northwest Research Institute, Seattle, Washington, USA.
Gareth A CromiePacific Northwest Research Institute, Seattle, Washington, USA.
Michelle TangPacific Northwest Research Institute, Seattle, Washington, USA.ORCID 0000-0002-8779-8697
Aimée M DudleyPacific Northwest Research Institute, Seattle, Washington, USA.ORCID 0000-0003-3644-0625

Funding

High resolution genetic dissection of complex and quantitative traits in yeastR01GM117119 · NIGMS · UNIVERSITY OF WASHINGTON · PI AKEY, JOSHUA MICHAEL, DUDLEY, AIMEE M · 2016 to 2019
$2.2M
NIGMS NIH HHS R01 GM117119NIH HHS
6 · The paper itself

Abstract

Meiotic mapping, a linkage-based method for analyzing the recombinant progeny of a cross, has long been a cornerstone of genetic research. The yeast Saccharomyces cerevisiae is a powerful system because it is possible to isolate and cultivate the four products (spores) of a single meiotic event. However, the throughput of this process has historically been limited by the process of identifying tetrads in a heterogeneous population of vegetative cells, tetrads, and dyads followed by manual separation (dissection) of the spores contained in a tetrad. To date, methods that facilitate high throughput characterization and isolation of meiotic progeny have relied on genetic engineering. Here, we characterize the ability of the fluorescent dye DiBAC

Indexed as

MeiosisSaccharomyces cerevisiaeFlow CytometrySpores, Fungalcoloring agentsflow cytometrymeiosisSaccharomyces cerevisiae

Identifiers

PMID35706372
PMCPMC9357172

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.