Evidence map›Paper›PMID 35705732›Full record

ArticleDigestive diseases and sciences2023

CCCTC-Binding Factor Mediates the Transcription of Insulin-Like Growth Factor Binding Protein 5 Through EZH2 in Ulcerative Colitis.

Dan Gu, Ting Cao, Shijie Yi, Ya Liu, Chao Fan

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Article in Digestive diseases and sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dan GuDepartment of Gastroenterology, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
Ting CaoDepartment of Gastroenterology, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
Shijie YiDepartment of Gastrointestinal Surgery, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421001, Hunan, People's Republic of China.
Ya LiuDepartment of Anorectal Surgery, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, 421001, Hunan, People's Republic of China.
Chao FanDepartment of Anorectal Surgery, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, 421001, Hunan, People's Republic of China. Fanchao491@163.com.ORCID http://orcid.org/0000-0001-9469-5402
University of South China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundUlcerative colitis (UC) features chronic, non-infectious inflammation of the colon. Insulin-like growth factor binding protein 5 (IGFBP5) has been indicated to be related to various inflammation-related diseases. However, its association with UC remains largely unclear.

aimsHere, we investigate the role of IGFBP5 in colonic mucosal epithelial cell injury in UC.

methodsDifferentially expressed genes in the colonic tissues of UC mice were screened using the Gene Expression Omnibus database, and IGFBP5 was identified. UC mice were developed using dextran sulfate sodium, and IGFBP5 expression in the colonic tissues of UC mice was confirmed by immunohistochemistry and RT-qPCR. The effects of IGFBP5 in vivo and in vitro were investigated by intraperitoneal injection of adeno-associated virus into UC mice or by transfection with an IGFBP5 overexpression plasmid into lipopolysaccharide-treated colonic mucosal epithelial cells. The mechanisms causing IGFBP5 deletion in UC were predicted by bioinformatics analysis and ChIP-qPCR and verified by rescue experiments.

resultsIGFBP5 was reduced in UC. IGFBP5 impaired the NFκB pathway in the colonic tissue of UC mice and ameliorated inflammatory infiltration and colonic mucosal cell injury. IGFBP5 depletion was associated with H3K27me3 modification, which was induced by EZH2. CTCF was responsible for recruiting EZH2 to the promoter region of IGFBP5. CTCF inhibition repressed UC progression by reducing H3K27me3 modification via the discouragement of the enrichment of EZH2 in the IGFBP5 promoter.

conclusionsCTCF modulates H3K27me3 modification of the IGFBP5 promoter by recruiting EZH2, thereby downregulating IGFBP5 to accentuate colonic mucosal epithelial cell injury in UC mice.

Indexed as

Colitis, UlcerativeAnimalsCarrier ProteinsCCCTC-Binding FactorEnhancer of Zeste Homolog 2 ProteinHistonesInflammationInsulin-Like Growth Factor Binding Protein 5MiceCarrier ProteinsCCCTC-Binding FactorCtcf protein, mouseEnhancer of Zeste Homolog 2 ProteinEzh2 protein, mouseHistonesIGFBP5-interacting protein, mouseInsulin-Like Growth Factor Binding Protein 5CTCFEZH2H3K27me3IGFBP5Ulcerative colitis

Identifiers

PMID35705732
OpenAlexW4282960947

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.