Evidence map›Paper›PMID 35705638›Full record

ArticleScientific data2022

Datasets for benchmarking antimicrobial resistance genes in bacterial metagenomic and whole genome sequencing.

Amogelang R Raphenya, James Robertson, Casper Jamin, Leonardo de Oliveira Martins, Finlay Maguire, Andrew G McArthur, John P Hays

Open access · goldAbstract readDataset
In one paragraph

Article in Scientific data, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

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  5. Microorganisms · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 6 institutions in 3 countries.

Amogelang R RaphenyaDavid Braley Centre for Antibiotic Discovery, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.
James RobertsonNational Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, N1G 3W4, Canada.ORCID 0000-0002-8534-5694
Casper JaminDepartment of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center, P. Debyelaan 25, 6229HX, Maastricht, the Netherlands.ORCID 0000-0003-0244-0467
Leonardo de Oliveira MartinsQuadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.ORCID 0000-0001-5247-1320
Finlay MaguireDepartment of Community Health & Epidemiology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
Andrew G McArthurDavid Braley Centre for Antibiotic Discovery, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.ORCID 0000-0002-1142-3063
John P HaysDepartment of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam (Erasmus MC), Doctor Molewaterplein 40, 3015 GD, Rotterdam, the Netherlands. j.hays@erasmusmc.nl.ORCID 0000-0001-9183-4497
McMaster University · CADalhousie University · CAErasmus MC · NLMaastricht University · NLNorwich Research Park · GBPublic Health Agency of Canada · CA

Funding

Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) PJT-156214Joint Programming Initiative on Antimicrobial Resistance (Joint Programming Initiative for Antimicrobial Resistance) 549010001Medical Research Council MR/L015080/1Medical Research Council MR/T030062/1Quadram Institute Bioscience BB/CCG1860/1
6 · The paper itself

Abstract

Whole genome sequencing (WGS) is a key tool in identifying and characterising disease-associated bacteria across clinical, agricultural, and environmental contexts. One increasingly common use of genomic and metagenomic sequencing is in identifying the type and range of antimicrobial resistance (AMR) genes present in bacterial isolates in order to make predictions regarding their AMR phenotype. However, there are a large number of alternative bioinformatics software and pipelines available, which can lead to dissimilar results. It is, therefore, vital that researchers carefully evaluate their genomic and metagenomic AMR analysis methods using a common dataset. To this end, as part of the Microbial Bioinformatics Hackathon and Workshop 2021, a 'gold standard' reference genomic and simulated metagenomic dataset was generated containing raw sequence reads mapped against their corresponding reference genome from a range of 174 potentially pathogenic bacteria. These datasets and their accompanying metadata are freely available for use in benchmarking studies of bacteria and their antimicrobial resistance genes and will help improve tool development for the identification of AMR genes in complex samples.

Indexed as

Anti-Bacterial AgentsBacteriaBenchmarkingDrug Resistance, BacterialGenome, BacterialMicrobial Sensitivity TestsWhole Genome SequencingAnti-Bacterial Agents

Identifiers

PMID35705638
PMCPMC9200708
OpenAlexW4282932896

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.