ArticleNPJ biofilms and microbiomes2022
Microrheology of Pseudomonas aeruginosa biofilms grown in wound beds.
Article in NPJ biofilms and microbiomes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- A technique to create hydrogels with tethered concentration gradients of moleculesSoft matter · 2026Article
- Resolving spatiotemporal dynamics in bacterial multicellular populations: approaches and challenges.Microbiology and molecular biology reviews : MMBR · 2025Review
- Speckle fluctuations reveal dynamics of microparticles in fibrin scaffolds in a model of bacterial infection.npj biological physics and mechanics · 2025Article
- Mind the gap: Exploring extracellular spaces in the brain with particle tracking and AI.Biophysical journal · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Emerging Issues and Initial Insights into Bacterial Biofilms: From Orthopedic Infection to Metabolomics.Antibiotics (Basel, Switzerland) · 2024Review
- Physiological Concentrations of Calcium Interact with Alginate and Extracellular DNA in the Matrices ofLangmuir : the ACS journal of surfaces and colloids · 2023Article
- Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection.Scientific reports · 2023Article
- Physiological concentrations of calcium interact with alginate and extracellular DNA in the matrices ofbioRxiv : the preprint server for biology · 2023Article
- Antimicrobial Resistance, Genetic Lineages, and Biofilm Formation inAntibiotics (Basel, Switzerland) · 2023Article
- Biofilms in Chronic Wound Infections: Innovative Antimicrobial Approaches Using the In Vitro Lubbock Chronic Wound Biofilm Model.International journal of molecular sciences · 2023Review
- Perspective: The viscoelastic properties of biofilm infections and mechanical interactions with phagocytic immune cells.Frontiers in cellular and infection microbiology · 2023Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A new technique was used to measure the viscoelasticity of in vivo Pseudomonas aeruginosa biofilms. This was done through ex vivo microrheology measurements of in vivo biofilms excised from mouse wound beds. To our knowledge, this is the first time that the mechanics of in vivo biofilms have been measured. In vivo results are then compared to typical in vitro measurements. Biofilms grown in vivo are more relatively elastic than those grown in a wound-like medium in vitro but exhibited similar compliance. Using various genetically mutated P. aeruginosa strains, it is observed that the contributions of the exopolysaccharides Pel, Psl, and alginate to biofilm viscoelasticity were different for the biofilms grown in vitro and in vivo. In vitro experiments with collagen containing medium suggest this likely arises from the incorporation of host material, most notably collagen, into the matrix of the biofilm when it is grown in vivo. Taken together with earlier studies that examined the in vitro effects of collagen on mechanical properties, we conclude that collagen may, in some cases, be the dominant contributor to biofilm viscoelasticity in vivo.
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