Evidence map›Paper›PMID 35705053›Full record

ArticleCell reports2022

Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.

Thanh Hoang, Dong Won Kim, Haley Appel, Nicole A Pannullo, Patrick Leavey, Manabu Ozawa, Sika Zheng, Minzhong Yu, Neal S Peachey, Seth Blackshaw

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 74 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Thanh HoangSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: vhoang3@jhmi.edu.
Dong Won KimSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: dkim195@jhmi.edu.
Haley AppelSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nicole A PannulloSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Patrick LeaveySolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Manabu OzawaCenter for Experimental Medicine and Systems Biology, University of Tokyo, Tokyo, Japan.
Sika ZhengDivision of Biomedical Sciences, University of California, Riverside, CA, USA.
Minzhong YuDepartment of Ophthalmic Research, Cole Eye Institute, Cleveland, OH, USA; Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA.
Neal S PeacheyDepartment of Ophthalmic Research, Cole Eye Institute, Cleveland, OH, USA; Department of Ophthalmology, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA.
Seth BlackshawSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: sblack@jhmi.edu.
Johns Hopkins University · USCleveland Clinic Lerner College of Medicine · USThe University of Tokyo · JPUniversity of California, Riverside · US

Funding

Use of systems pharmacology to prevent rod and cone photoreceptor degenerationR24EY027283 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI PALCZEWSKI, KRZYSZTOF · 2017 to 2021
$7.9M
RESOURCE/SERVICE CORE C - MOLECULAR INFORMATICS MODULEP30EY025585 · NEI · CLEVELAND CLINIC LERNER COM-CWRU · PI BELA ANAND-APTE · 2016 to 2026
$7.7M
Transcriptional regulation of retinal cell differentiation and functionR01EY020560 · NEI · JOHNS HOPKINS UNIVERSITY · PI BLACKSHAW, SETH · 2011 to 2021
$4.4M
Comparative transcriptomic and epigenomic analyses of Muller glia reprogrammingU01EY027267 · NEI · UNIVERSITY OF NOTRE DAME · PI BLACKSHAW, SETH, HYDE, DAVID R · 2016 to 2019
$3.0M
The Role of NMD in Cortical Neural Progenitor CellsR01NS125276 · NINDS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Sika Zheng · 2022 to 2026
$2.6M
RNA regulation of intrinsically-determined neuronal propertiesR01MH116220 · NIMH · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI ZHENG, SIKA · 2018 to 2022
$2.4M
The Regulation and Function of Neuron-Specific Alternative SplicingR01NS104041 · NINDS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI ZHENG, SIKA · 2018 to 2022
$2.1M
Identification of gene regulatory networks that control proliferative and neurogenic competence in mammalian Müller gliaR01EY031685 · NEI · JOHNS HOPKINS UNIVERSITY · PI BLACKSHAW, SETH · 2020 to 2023
$2.0M
NEI NIH HHS P30 EY025585NEI NIH HHS R01 EY020560NEI NIH HHS R01 EY031685NEI NIH HHS R24 EY027283NEI NIH HHS U01 EY027267NIMH NIH HHS R01 MH116220NINDS NIH HHS R01 NS104041NINDS NIH HHS R01 NS125276
6 · The paper itself

Abstract

Direct reprogramming of glia into neurons is a potentially promising approach for the replacement of neurons lost to injury or neurodegenerative disorders. Knockdown of the polypyrimidine tract-binding protein Ptbp1 has been recently reported to induce efficient conversion of retinal Mϋller glia into functional neurons. Here, we use a combination of genetic lineage tracing, single-cell RNA sequencing (scRNA-seq), and electroretinogram analysis to show that selective induction of either heterozygous or homozygous loss-of-function mutants of Ptbp1 in adult retinal Mϋller glia does not lead to any detectable level of neuronal conversion. Only a few changes in gene expression are observed in Mϋller glia following Ptbp1 deletion, and glial identity is maintained. These findings highlight the importance of using genetic manipulation and lineage-tracing methods in studying cell-type conversion.

Indexed as

Heterogeneous-Nuclear RibonucleoproteinsPolypyrimidine Tract-Binding ProteinNeurogliaNeuronsRetinaHeterogeneous-Nuclear RibonucleoproteinsPolypyrimidine Tract-Binding Proteincell reprogrammingCP: Cell biologyglia-to-neuron conversionMϋller gliaPtbp1PTBP1regenerationretinaretinal ganglion cellRNA splicingtransdifferentiation

Identifiers

PMID35705053
PMCPMC9619396
OpenAlexW4282823804

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.