ArticleScientific reports2022
Monitoring and imaging pH in biofilms utilizing a fluorescent polymeric nanosensor.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Validated workflows for preparing and characterizing core-stained and surface-labeled fluorescent polymer particles with simple commercial automation tools.Analytical and bioanalytical chemistry · 2026Article
- Stimuli-Responsive Nanomaterial-Based Biosensor Structures for Wound Care: pH, ROS, and Temperature Sensing Strategies.Micromachines · 2026Review
- Recombinant Expression and Bioprocess Optimization of Priestia megaterium α-Amylase and Its Impact on Dough Fermentation Efficiency.Chemistry & biodiversity · 2025Article
- Gram Negative Biofilms: Structural and Functional Responses to Destruction by Antibiotic-Loaded Mixed Polymeric Micelles.Microorganisms · 2024Article
- Advanced Imaging Methodology in Bacterial Biofilms with a Fluorescent Enzymatic Sensor for pepN Activity.Biosensors · 2024Article
- Artificial Intelligence-Driven Analysis of Antimicrobial-Resistant and Biofilm-Forming Pathogens on Biotic and Abiotic Surfaces.Antibiotics (Basel, Switzerland) · 2024Review
- A targeted fluorescent nanosensor for ratiometric pH sensing at the cell surface.Scientific reports · 2024Article
- Innovative Phospholipid Carriers: A Viable Strategy to Counteract Antimicrobial Resistance.International journal of molecular sciences · 2023Review
- Microbiologically influenced corrosion-more than just microorganisms.FEMS microbiology reviews · 2023Review
- Influence of nanoparticle encapsulation and encoding on the surface chemistry of polymer carrier beads.Scientific reports · 2023Article
- Efficiency scale for scattering luminescent particles linked to fundamental and measurable spectroscopic properties.Scientific reports · 2023Article
- ROS generating BODIPY loaded nanoparticles for photodynamic eradication of biofilms.Frontiers in microbiology · 2023Article
- Review
- Advances in non-invasive biosensing measures to monitor wound healing progression.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms are ubiquitous in nature and in the man-made environment. Given their harmful effects on human health, an in-depth understanding of biofilms and the monitoring of their formation and growth are important. Particularly relevant for many metabolic processes and survival strategies of biofilms is their extracellular pH. However, most conventional techniques are not suited for minimally invasive pH measurements of living biofilms. Here, a fluorescent nanosensor is presented for ratiometric measurements of pH in biofilms in the range of pH 4.5-9.5 using confocal laser scanning microscopy. The nanosensor consists of biocompatible polystyrene nanoparticles loaded with pH-inert dye Nile Red and is surface functionalized with a pH-responsive fluorescein dye. Its performance was validated by fluorometrically monitoring the time-dependent changes in pH in E. coli biofilms after glucose inoculation at 37 °C and 4 °C. This revealed a temperature-dependent decrease in pH over a 4-h period caused by the acidifying glucose metabolism of E. coli. These studies demonstrate the applicability of this nanosensor to characterize the chemical microenvironment in biofilms with fluorescence methods.
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