ArticleJournal of veterinary science2022
Development of a ladder-shape melting temperature isothermal amplification (LMTIA) assay for detection of African swine fever virus (ASFV).
Article in Journal of veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The trial behind it
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Triplex Proofman-LMTIA: A Rapid, Specific, and Sensitive Assay for Detecting Wheat, Peanut, and Soybean Allergens in Foods.Foods (Basel, Switzerland) · 2026Article
- The ladder-shape melting temperature isothermal amplification methods for detection of CaMV 35S and CP4 EPSPS genes in soybean foods.Transgenic research · 2026Article
- Development of Real-Time and Lateral Flow Dipstick Recombinase Polymerase Amplification Assays for the Rapid Field Diagnosis of MGF-505R Gene-Deleted Mutants of African Swine Fever Virus.Veterinary sciences · 2025Article
- Development and evaluation of a colorimetric LAMP-based biosensor for rapid detection of a nosocomial infection agent, Citrobacter freundii.Scientific reports · 2023Article
- Rapid Discrimination ofMolecules (Basel, Switzerland) · 2023Article
- Detection ofMolecules (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDue to the unavailability of an effective vaccine or antiviral drug against the African swine fever virus (ASFV), rapid diagnosis methods are needed to prevent highly contagious African swine fever.
objectivesThe objective of this study was to establish the ladder-shape melting temperature isothermal amplification (LMTIA) assay for the detection of ASFV.
methodsLMTIA primers were designed with the p72 gene of ASFV as the target, and plasmid pUC57 was used to clone the gene. The LMTIA reaction system was optimized with the plasmid as the positive control, and the performance of the LMTIA assay was compared with that of the commercial real-time polymerase chain reaction (PCR) kit in terms of sensitivity and detection rate using 200 serum samples.
resultsOur results showed that the LMTIA assay could detect the 10
conclusionsThe LMTIA assay had high sensitivity, good detection, and simple operation. Thus, it is suitable for facilitating preliminary and cost-effective surveillance for the prevention and control of ASFV.
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