ArticleClinical and experimental reproductive medicine2022
Melatonin and selenium supplementation in extenders improves the post-thaw quality parameters of rat sperm.
Article in Clinical and experimental reproductive medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Mechanistic understanding of heat stress in cattle reproduction toward pharmacological strategies: review.Animal bioscience · 2026Article
- L-Selenomethionine Alleviates Cryo-Induced Ferroptosis Through the NRF2-SLC7A11-GPX4 Pathway, Improving Post-Thaw In Vitro Quality of Dairy Goat Spermatozoa.Antioxidants (Basel, Switzerland) · 2026Article
- Melatonin: A Silver Bullet for Fertility and Reproductive Health.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm.Animals : an open access journal from MDPI · 2024Article
- The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification.Clinical and experimental reproductive medicine · 2024Article
- Evaluation of the effect of mitoquinone on functional parameters, DNA structure, and genes expression related to the apoptotic and antioxidants of human sperm after freezing-thawing.Molecular biology reports · 2024Article
- Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing.Veterinary sciences · 2023Article
- Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos.Current issues in molecular biology · 2022Review
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to determine the effects of melatonin and selenium in freezing extenders on frozen-thawed rat sperm.
methodsSemen samples were collected from 20 adult male Wistar albino rats. Following dilution, the samples were divided into six groups: four cryopreserved groups with 1 mM and 0.5 mM melatonin and selenium supplements, and two fresh and cryopreserved control groups. The rapid freezing technique was used to freeze the samples. Flow cytometry was used to assess plasma membrane integrity, mitochondrial membrane potential, and DNA damage, while computer-assisted sperm analysis was used to assess motility.
resultsTotal motility was higher in the 1 mM melatonin supplementation group than in the cryopreserved control group (mean±standard error of the mean, 69.89±3.05 vs. 59.21±1.31; p≤0.05). The group with 1 mM selenium had the highest plasma membrane integrity (42.35%±1.01%). The cryopreserved group with 0.5 mM selenium had the highest mitochondrial membrane potential, whereas the cryopreserved control group had the lowest (45.92%±4.53% and 39.45%±3.52%, respectively).
conclusionCryopreservation of rat semen supplemented with 1 mM melatonin increased sperm motility after freeze-thawing, while supplementation with 0.5 mM selenium increased mitochondrial activity.
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