Evidence map›Paper›PMID 35698377›Full record

ArticleCarbohydrate polymers2022

Lyophilization induces physicochemical alterations in cryptococcal exopolysaccharide.

Maggie P Wear, Audra A Hargett, John E Kelly, Scott A McConnell, Conor J Crawford, Darón I Freedberg, Ruth E Stark, Arturo Casadevall

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Carbohydrate polymers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Neutron Scattering Analysis ofBiomacromolecules · 2024
    Article
  6. Methods of Cryptococcal Polysaccharide Analysis Using ELISA.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  7. Neutron Scattering Analysis ofbioRxiv : the preprint server for biology · 2023
    Article
  8. The hypothermic nature of fungi.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Maggie P WearW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. Electronic address: mwear1@jhu.edu.
Audra A HargettLaboratory of Bacterial PSs, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.
John E KellyDepartment of Chemistry and Biochemistry, The City College of New York and CUNY Institute for Macromolecular Assemblies, New York, NY, USA.
Scott A McConnellW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Conor J CrawfordW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Darón I FreedbergLaboratory of Bacterial PSs, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.
Ruth E StarkDepartment of Chemistry and Biochemistry, The City College of New York and CUNY Institute for Macromolecular Assemblies, New York, NY, USA; Ph.D. Programs in Chemistry and Biochemistry, The City University of New York, New York, NY, USA.
Arturo CasadevallW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Funding

Training: Molecular & Cellular Bases of Infectious DiseasesT32AI007417 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Arturo Casadevall · 1994 to 2026
$11.9M
Biology of Fungal MelaninR01AI052733 · NIAID · YESHIVA UNIVERSITY · PI CASADEVALL, ARTURO · 2003 to 2019
$6.6M
Conjugate vaccines for prevention and treatment of cryptococcosis - COVID-19 Revision SupplementR01AI152078 · NIAID · JOHNS HOPKINS UNIVERSITY · PI CASADEVALL, ARTURO · 2020 to 2024
$5.1M
NIAID NIH HHS R01 AI052733NIAID NIH HHS R01 AI152078NIAID NIH HHS T32 AI007417
6 · The paper itself

Abstract

Microbial polysaccharide characterization requires purification that often involves detergent precipitation and lyophilization. Here we examined physicochemical changes following lyophilization of Cryptococcus neoformans exopolysaccharide (EPS). Solution

Indexed as

Cryptococcus neoformansPolysaccharidesFreeze DryingMagnetic Resonance SpectroscopyMicroscopy, Electron, TransmissionPolysaccharides, BacterialPolysaccharidesPolysaccharides, BacterialCryptococcusDLSExopolysaccharideLyophilizationNMRTEM

Identifiers

PMID35698377
PMCPMC10064552

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.