ArticleNature biotechnology2022
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping.
Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 92 citations in OpenAlex.
- Depth-adapted adaptive optics for three-photon microscopy.Biomedical optics express · 2026Article
- Multispectral photoacoustic microscopy and NIR-II fluorescence imaging of TREM2-positive microglia in Aβ-driven Alzheimer's pathogenesis.Fundamental research · 2026Article
- Real-Time In Vivo Cellular-Level Imaging During Puncture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Large field-of-view volumetric deep brain imaging through gradient-index lenses.Nature communications · 2025Article
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging.Nature communications · 2025Article
- Optical focusing into scattering media via iterative time reversal guided by absorption nonlinearity.Nature communications · 2025Article
- Compressive Fourier-Domain Intensity Coupling (C-FOCUS) enables near-millimeter deep imaging in the intact mouse brain in vivo.Research square · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Molecular Gold Nanoclusters for Advanced NIR-II Bioimaging and Therapy.Chemical reviews · 2025Review
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Line-scan chromatic confocal microscopy for multi-depth imaging of skin tissue using deconvolution.Biomedical optics express · 2025Article
- Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.Communications biology · 2025Article
- Optical multi-beam steering and communication using integrated acousto-optics arrays.Nature communications · 2025Article
- Deep-Penetrating and High-Resolution Continuous-Wave Nonlinear Microscopy Based on Homologous Dual-Emission Upconversion Adaptive Optics.Nano letters · 2025Article
- Scattering correction through Fourier-domain intensity coupling in two-photon microscopy (2P-FOCUS).Photonics research · 2025Article
- The Environmental Impact of Medical Imaging Agents and the Roadmap to Sustainable Medical Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.iScience · 2024Article
- Advances in non-invasive imaging of proteinopathies in animal models of neurodegenerative diseases.Neural regeneration research · 2024Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution optical imaging deep in tissues is challenging because of optical aberrations and scattering of light caused by the complex structure of living matter. Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering induced by specimens and recovers subcellular resolution at depth. A conjugate adaptive optics configuration with remote focusing enables in vivo imaging of fine neuronal structures in the mouse cortex through the intact skull up to a depth of 750 µm below the pia, enabling near-non-invasive high-resolution microscopy in cortex. Functional calcium imaging with high sensitivity and high-precision laser-mediated microsurgery through the intact skull were also demonstrated. Moreover, we achieved in vivo high-resolution imaging of the deep cortex and subcortical hippocampus up to 1.1 mm below the pia within the intact brain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.