Evidence map›Paper›PMID 35697796›Full record

ArticleNature microbiology2022

Mating pair stabilization mediates bacterial conjugation species specificity.

Wen Wen Low, Joshua L C Wong, Leticia C Beltran, Chloe Seddon, Sophia David, Hok-Sau Kwong, Tatiana Bizeau, Fengbin Wang, Alejandro Peña, Tiago R D Costa and 5 more

Open access · hybridAbstract read
In one paragraph

Article in Nature microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed
14.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 100 citations in OpenAlex.

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  20. Fluid flow generates bacterial conjugation hot spots by increasing the rate of shear-driven cell-cell encounters.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Wen Wen LowMRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK.ORCID http://orcid.org/0000-0003-4004-7244
Joshua L C Wong *MRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK.ORCID http://orcid.org/0000-0001-8437-6731
Leticia C Beltran *Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.
Chloe Seddon *MRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK.
Sophia David *Centre for Genomic Pathogen Surveillance, Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, UK.
Hok-Sau KwongDepartment of Life Sciences, Imperial College, London, UK.
Tatiana BizeauDepartment of Life Sciences, Imperial College, London, UK.
Fengbin WangDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.ORCID http://orcid.org/0000-0003-1008-663X
Alejandro PeñaMRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK.
Tiago R D CostaMRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK.ORCID http://orcid.org/0000-0003-2716-9669
Bach PhamDepartment of Chemistry, University of Massachusetts Amherst, Amherst, MA, USA.
Min ChenDepartment of Chemistry, University of Massachusetts Amherst, Amherst, MA, USA.
Edward H EgelmanDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA.ORCID http://orcid.org/0000-0003-4844-5212
Konstantinos BeisDepartment of Life Sciences, Imperial College, London, UK.ORCID http://orcid.org/0000-0001-5727-4721
Gad FrankelMRC Centre for Molecular Microbiology and Infection, Imperial College, London, UK. g.frankel@imperial.ac.uk.ORCID http://orcid.org/0000-0002-0046-1363
Imperial College London · GBUniversity of Virginia · USRutherford Appleton Laboratory · GBUniversity of Massachusetts Amherst · USUniversity of Oxford · GB

Funding

Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic ResolutionR35GM122510 · NIGMS · UNIVERSITY OF VIRGINIA · PI EDWARD H. EGELMAN · 2017 to 2026
$7.3M
UVA molecular electron microscopy core for the Mid-Atlantic regionU24GM116790 · NIGMS · UNIVERSITY OF VIRGINIA · PI GALKIN, VITOLD, SAMSO, MONTSERRAT · 2017 to 2021
$2.9M
Molecular Electron Microscopy Core Facility ImprovementsG20RR031199 · NCRR · UNIVERSITY OF VIRGINIA · PI SHUPNIK, MARGARET A · 2010 to 2010
$2.0M
300 keV Liquid Helium Robotic MicroscopeS10RR025067 · NCRR · UNIVERSITY OF VIRGINIA · PI EGELMAN, EDWARD H. · 2009 to 2009
$2.0M
Biotechnology and Biological Sciences Research Council BB/M011178/1Medical Research Council MR/N020103/1Medical Research Council MR/R502376/1NCRR NIH HHS G20 RR031199NCRR NIH HHS S10 RR025067NIGMS NIH HHS R35 GM122510NIGMS NIH HHS U24 GM116790Wellcome TrustWellcome Trust 107057/Z/15/Z
6 · The paper itself

Abstract

Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread of virulence and resistance plasmids. Here we describe how variants of the plasmid-encoded donor outer membrane (OM) protein TraN cooperate with distinct OM receptors in recipients to mediate mating pair stabilization and efficient DNA transfer. We show that TraN from the plasmid pKpQIL (Klebsiella pneumoniae) interacts with OmpK36, plasmids from R100-1 (Shigella flexneri) and pSLT (Salmonella Typhimurium) interact with OmpW, and the prototypical F plasmid (Escherichia coli) interacts with OmpA. Cryo-EM analysis revealed that TraN

Indexed as

Bacterial ProteinsConjugation, GeneticEscherichia coliF FactorPorinsSpecies SpecificityBacterial ProteinsPorins

Identifiers

PMID35697796
PMCPMC9246713
OpenAlexW4282582526

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.