ArticleNature cell biology2022
Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.
Article in Nature cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 124 papers.
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Who cites it
124 citing papers in PubMed, 180 citations in OpenAlex.
- RAFTER: a releasing factor tethered RNA editing system for quantifying mRNA translation.Life science alliance · 2026Article
- Protocol for ribosome-protected fragment isolation from rare leukemia cell populations.STAR protocols · 2026Article
- Phase Separation of SF3B1 Serves as a Critical Post-Transcriptional Regulator During Early Mouse Embryogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- CPEB1 orchestrates cell cycle-dependent translation of maternal factors to coordinate nuclear-cytoplasmic maturation in porcine oocytes.Science China. Life sciences · 2026Article
- Regulation of mRNA poly(A) tail length governs mammalian body plan formation in gastruloids.Nature communications · 2026Article
- Asymmetric RBBP7 regulates the first cell fate decision of early mammalian embryos.Cell discovery · 2026Article
- High-resolution mapping of embryonic genome activation unveils a decoupling of transcription activation from precocious H3K4me3 removal.Science advances · 2026Article
- Subcellularly Resolved 3D Translatome in Mouse Oocytes and Early Embryos.bioRxiv : the preprint server for biology · 2026Article
- Spaceflight multi-omics reveals vulnerabilities of human germ cell development.Science advances · 2026Article
- The degradation of maternal RNA-binding protein 4E-T regulates translational activation to ensure maternal-to-zygotic transition in mouse embryos.Cellular and molecular life sciences : CMLS · 2026Article
- Proteomics-based insights into mammalian oocyte and early embryo development.Nature cell biology · 2026Review
- Dynamic regulation of H3K9 dimethylation drives mouse minor zygotic genome activation.Nature structural & molecular biology · 2026Article
- PIWIL3-piRNA pathway controls rabbit oogenesis and embryogenesis via broad regulation of the transcriptome and proteome.Nature communications · 2026Article
- TENT-5 polyadenylates and regulates male-specific transcripts in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2026Article
- Pre-implantation embryo metabolism identified by PEMA reveals endogenous lactate insufficiency contributes to pre-implantation development arrest.Fundamental research · 2026Article
- Deciphering tRNA repertoires and translation coordination during mouse early embryogenesis by ORACLE-tRNAseq.Nature communications · 2026Article
- U2AF1 modulates alternative exon selection and guards zygotic splicing activation in mouse preimplantation embryogenesis.Cellular and molecular life sciences : CMLS · 2026Article
- Single-oocyte full-length isoform sequencing unveils the impact of transposable elements on RNA diversity and stability.Nature communications · 2026Article
- Oocyte vitrification disrupts zygotic genome activation in embryos by impairing maternal spliceosome translation and Crxos splicing.PLoS genetics · 2026Article
- mThe EMBO journal · 2026Article
64 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
23 authors at 9 institutions in 2 countries.
Funding
Abstract
In mammals, translational control plays critical roles during oocyte-to-embryo transition (OET) when transcription ceases. However, the underlying regulatory mechanisms remain challenging to study. Here, using low-input Ribo-seq (Ribo-lite), we investigated translational landscapes during OET using 30-150 mouse oocytes or embryos per stage. Ribo-lite can also accommodate single oocytes. Combining PAIso-seq to interrogate poly(A) tail lengths, we found a global switch of translatome that closely parallels changes of poly(A) tails upon meiotic resumption. Translation activation correlates with polyadenylation and is supported by polyadenylation signal proximal cytoplasmic polyadenylation elements (papCPEs) in 3' untranslated regions. By contrast, translation repression parallels global de-adenylation. The latter includes transcripts containing no CPEs or non-papCPEs, which encode many transcription regulators that are preferentially re-activated before zygotic genome activation. CCR4-NOT, the major de-adenylation complex, and its key adaptor protein BTG4 regulate translation downregulation often independent of RNA decay. BTG4 is not essential for global de-adenylation but is required for selective gene de-adenylation and production of very short-tailed transcripts. In sum, our data reveal intimate interplays among translation, RNA stability and poly(A) tail length regulation underlying mammalian OET.
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