Evidence map›Paper›PMID 35696489›Full record

ReviewJournal of cellular physiology2022

Extending applications of AFM to fluidic AFM in single living cell studies.

Yuan Qiu, Chen-Chi Chien, Basile Maroulis, Jiani Bei, Angelo Gaitas, Bin Gong

Open access · greenAbstract readReview
In one paragraph

Review in Journal of cellular physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yuan QiuDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Chen-Chi ChienThe Estelle and Daniel Maggin Department of Neurology, Icahn School of Medicine at Mount Sinai, New York City, New York, USA.
Basile MaroulisThe Estelle and Daniel Maggin Department of Neurology, Icahn School of Medicine at Mount Sinai, New York City, New York, USA.
Jiani BeiDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Angelo GaitasThe Estelle and Daniel Maggin Department of Neurology, Icahn School of Medicine at Mount Sinai, New York City, New York, USA.
Bin GongDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
The University of Texas Medical Branch at Galveston · USIcahn School of Medicine at Mount Sinai · USHess (United States) · US

Funding

Epac1 Plays a Critical Role in Bacterial Adhesion during RickettsiosesR01AI121012 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2016 to 2020
$1.9M
Targeting the Host Adenosine A2A Receptor to Protect Against Fatal Rickettsiosis Using an Approved Parkinson's Disease DrugR21AI137785 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2018 to 2019
$440k
The role of IL-1 family of cytokines in tick-transmitted ehrlichiosisR21AI144328 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2019 to 2020
$435k
Targeting extracellular tRNA-derived RNA fragments (tRFs) to protect against fatal rickettsiosisR21AI154211 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2020 to 2021
$435k
Inflammasome activation and ehrlichial dissemination in tick vs needle transmissionR03AI142406 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GONG, BIN · 2019 to 2020
$158k
NIAID NIH HHS R01 AI121012NIAID NIH HHS R03 AI142406NIAID NIH HHS R21 AI137785NIAID NIH HHS R21 AI144328NIAID NIH HHS R21 AI154211Sealy Center for Vector Borne and Zoonotic Diseases pilot grant FY21 (BG)UTMB Center for Biodefense and Emerging Infectious Diseases pilot grant FY21 (BG)
6 · The paper itself

Abstract

In this article, a review of a series of applications of atomic force microscopy (AFM) and fluidic Atomic Force Microscopy (fluidic AFM, hereafter fluidFM) in single-cell studies is presented. AFM applications involving single-cell and extracellular vesicle (EV) studies, colloidal force spectroscopy, and single-cell adhesion measurements are discussed. FluidFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. In this review, capacities of fluidFM are discussed to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces of endothelial or epithelial cells in a confluent cell monolayer in an appropriate physiological environment, and (3) the ease of measurement of vertical binding forces of intercellular adhesion between heterogeneous cells. Furthermore, key applications of fluidFM are reviewed regarding to EV absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

Indexed as

MicrofluidicsMicroscopy, Atomic ForceSingle-Cell AnalysisCell AdhesionCell CountEndothelial CellsEpithelial CellsExtracellular Vesiclesatomic force microscopyextracellular vesiclefluidic atomic force microscopysingle living cell

Identifiers

PMID35696489
PMCPMC9378449
OpenAlexW4282573956

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.