Evidence map›Paper›PMID 35696356›Full record

ArticlePloS one2022

NADPH-oxidase 4 gene over-expression in peripheral blood lymphocytes of the schizophrenia patients.

Elizaveta S Ershova, Galina V Shmarina, Andrey V Martynov, Natalia V Zakharova, Roman V Veiko, Pavel E Umriukhin, George P Kostyuk, Sergey I Kutsev, Natalia N Veiko, Svetlana V Kostyuk

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PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Elizaveta S ErshovaResearch Centre for Medical Genetics, Moscow, Russia.
Galina V ShmarinaResearch Centre for Medical Genetics, Moscow, Russia.
Andrey V MartynovResearch Centre for Medical Genetics, Moscow, Russia.
Natalia V ZakharovaN. A. Alexeev Clinical Psychiatric Hospital №1, Moscow Healthcare Department, Moscow, Russia.
Roman V VeikoResearch Centre for Medical Genetics, Moscow, Russia.
Pavel E UmriukhinResearch Centre for Medical Genetics, Moscow, Russia.ORCID 0000-0001-8628-7353
George P KostyukN. A. Alexeev Clinical Psychiatric Hospital №1, Moscow Healthcare Department, Moscow, Russia.
Sergey I KutsevResearch Centre for Medical Genetics, Moscow, Russia.
Natalia N VeikoResearch Centre for Medical Genetics, Moscow, Russia.
Svetlana V KostyukResearch Centre for Medical Genetics, Moscow, Russia.
Research Centre for Medical Genetics · RUMoscow Research and Clinical Center for Neuropsychiatry · RUSechenov University · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionIncreased systemic oxidative stress is common in schizophrenia (SZ) patients. NADPH-oxidase 4 (NOX4) is the cell oxidoreductase, catalyzing the hydrogen peroxide formation. Presumably, NOX4 is the main oxidative stress factor in a number of diseases such as cardiovascular diseases and cancer. We hypothesized that NOX4 may be involved in the oxidative stress development caused by the disease in the schizophrenic patients' peripheral blood lymphocytes (PBL). MATERIALS AND

methodsThe SZ group included 100 patients (68 men and 32 women aged 28 ± 11 years). The control group included 60 volunteers (35 men and 25 women aged 25 ± 12 years). Flow cytometry analysis (FCA) was used for DNA damage markers (8-oxodG, ɣH2AX), pro- and antiapoptotic proteins (BAX1 and BCL2) and the master-regulator of anti-oxidant response NRF2 detection in the lymphocytes of the untreated SZ patients (N = 100) and the healthy control (HC, N = 60). FCA and RT-qPCR were used for NOX4 and RNANOX4 detection in the lymphocytes. RT-qPCR was used for mtDNA quantitation in peripheral blood mononuclear cells. Cell-free DNA concentration was determined in blood plasma fluorimetrically.

results8-oxodG, NOX4, and BCL2 levels in the PBL in the SZ group were higher than those in the HC group (p < 0.001). ɣH2AX protein level was increased in the subgroup with high 8-oxodG (p<0.02) levels and decreased in the subgroup with low 8-oxodG (p <0.0001) levels. A positive correlation was found between 8-oxodG, ɣH2AX and BAX1 levels in the SZ group (p <10-6). NOX4 level in lymphocytes did not depend on the DNA damage markers values and BAX1 and BCL2 proteins levels. In 15% of PBL of the HC group a small cellular subfraction was found (5-12% of the total lymphocyte pool) with high DNA damage level and elevated BAX1 protein level. The number of such cells was maximal in PBL samples with low NOX4 protein levels.

conclusionSignificant NOX4 gene expression was found a in SZ patients' lymphocytes, but the corresponding protein is probably not a cause of the DNA damage.

Indexed as

Schizophrenia8-Hydroxy-2'-DeoxyguanosineFemaleHumansLeukocytes, MononuclearLymphocytesMaleNADPNADPH Oxidase 4Oxidative StressProto-Oncogene Proteins c-bcl-28-Hydroxy-2'-DeoxyguanosineNADPNADPH Oxidase 4NOX4 protein, humanProto-Oncogene Proteins c-bcl-2

Identifiers

PMID35696356
PMCPMC9191697
OpenAlexW4282588271

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.