Evidence map›Paper›PMID 35696207›Full record

ArticleJournal of food and drug analysis2021

Development of the species-specific multiplex PCR and DNA sequencing methods for rapid authentication of Isatidis Folium and its adulterants.

Yung-Chuan Hsieh, Ming-Sian Wu, Hui-Chun Lee, Chia-Yun Hsieh, Shih-Shan Huang, Chia-Fen Tsai, Ya-Tze Lin, Mei-Chih Lin, Su-Hsiang Tseng, Der-Yuan Wang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of food and drug analysis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yung-Chuan HsiehDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Ming-Sian WuDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Hui-Chun LeeDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Chia-Yun HsiehDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Shih-Shan HuangDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Chia-Fen TsaiSouthern Center for Regional Administration, Food and Drug Administration, Ministry of Health and Welfare, No.180, Zihyou 2nd Rd., Zuoying Dist., Kaohsiung 81358, Taiwan.
Ya-Tze LinDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Mei-Chih LinDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Su-Hsiang TsengDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Der-Yuan WangDivision of Research and Analysis, Food and Drug Administration, Ministry of Health and Welfare, No.161-2 Kunyang St., Nangang Dist., Taipei 11561, Taiwan.
Ministry of Health and Welfare · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isatis indigotica Fort. (family Cruciferae), is an herb widely used in traditional herbal medicine and its dried leave was named "ISATIDIS FOLIUM". Baphicacanthus cusia (Ness) Bremek. and Polygonum tinctorium Ait. are commonly misused as ISATIDIS FOLIUM in Chinese Medicine pharmacy. For the purpose of being not misused, specific primers based on the sequence difference of chloroplast trnH-psbA intergenic spacer were designed and multiplex polymerase chain reaction method (multiplex PCR) was developed. In this study, 29 original herbal materials were analyzed and our results show that DNA size after multiplex PCR was able to distinguish variations between three herbs. DNA fragments of 464, 297, 170 base pairs (bps) were represented for I. indigotica and B. cusia and P. tinctorium, respectively. In conclusion, our investigations demonstrate that molecular identification method provides more accurate results for medicinal plants detection and good quality control of ISATIDIS FOLIUM.

Indexed as

IsatisPlants, MedicinalMultiplex Polymerase Chain ReactionPlant LeavesSequence Analysis, DNASpecies Specificity

Identifiers

PMID35696207
PMCPMC9261822
OpenAlexW3172543303

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.