ArticleFrontiers in microbiology2022
A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- ENVnet provides a global molecular resource of dissolved organic matter.Nature biotechnology · 2026Article
- Functional and ecological drivers of bacterial interactions in glacier-fed stream biofilms.mSystems · 2026Article
- Limits of bacterial osmoadaptation during planktonic and biofilm growth: a step toward effective biofouling control.Applied and environmental microbiology · 2026Article
- Metabolic blueprints of monocultures enable prediction and design of synthetic microbial consortia.bioRxiv : the preprint server for biology · 2026Article
- Interaction of plant-derived metabolites and rhizobiome functions enhances drought stress tolerance.Genome biology · 2025Article
- Breaking the reproducibility barrier with standardized protocols for plant-microbiome research.PLoS biology · 2025Article
- Low-cost in-house re-formulated brain heart infusion medium for effective planktonic growth and early detection of bloodstream bacterial pathogens.Frontiers in microbiology · 2025Article
- Rhizospheric bacteria from the Atacama Desert hyper-arid core: cultured community dynamics and plant growth promotion.Microbiology spectrum · 2024Article
- Soil metabolomics: Deciphering underground metabolic webs in terrestrial ecosystems.Eco-Environment & Health · 2024Review
- Top-down and bottom-up microbiome engineering approaches to enable biomanufacturing from waste biomass.Journal of industrial microbiology & biotechnology · 2024Review
- Molecular mechanisms and environmental adaptations of flagellar loss and biofilm growth of Rhodanobacter under environmental stress.The ISME journal · 2024Article
- Reproducible growth ofScience advances · 2024Article
- Developing stable, simplified, functional consortia fromFrontiers in microbiology · 2024Article
- Isolation and Identification of Culturable Bacteria from South China Seawater and Preliminary Screening of Marine Biocontrol Bacteria.Microorganisms · 2023Article
- Impact of Plant-Beneficial Bacterial Inocula on the Resident Bacteriome: Current Knowledge and Future Perspectives.Microorganisms · 2022Review
- Pseudomonas cultivated from Andropogon gerardii rhizosphere show functional potential for promoting plant host growth and drought resilience.BMC genomics · 2022Article
- Culturomics of Bacteria from Radon-Saturated Water of the World's Oldest Radium Mine.Microbiology spectrum · 2022Article
- Substrate Utilization and Competitive Interactions Among Soil Bacteria Vary With Life-History Strategies.Frontiers in microbiology · 2022Article
- Perspective: Simple State Communities to Study Microbial Interactions: Examples and Future Directions.Frontiers in microbiology · 2022Article
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15 authors.
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Abstract
Exometabolomics is an approach to assess how microorganisms alter, or react to their environments through the depletion and production of metabolites. It allows the examination of how soil microbes transform the small molecule metabolites within their environment, which can be used to study resource competition and cross-feeding. This approach is most powerful when used with defined media that enable tracking of all metabolites. However, microbial growth media have traditionally been developed for the isolation and growth of microorganisms but not metabolite utilization profiling through Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Here, we describe the construction of a defined medium, the Northen Lab Defined Medium (NLDM), that not only supports the growth of diverse soil bacteria but also is defined and therefore suited for exometabolomic experiments. Metabolites included in NLDM were selected based on their presence in R2A medium and soil, elemental stoichiometry requirements, as well as knowledge of metabolite usage by different bacteria. We found that NLDM supported the growth of 108 of the 110 phylogenetically diverse (spanning 36 different families) soil bacterial isolates tested and all of its metabolites were trackable through LC-MS/MS analysis. These results demonstrate the viability and utility of the constructed NLDM medium for growing and characterizing diverse microbial isolates and communities.
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