Evidence map›Paper›PMID 35690839›Full record

ArticleCell & bioscience2022

Both prokaryotes and eukaryotes produce an immune response against plasmids with 5'-GTTTGTT-3'.

Nan Li, Dongya Jiang, Luqingqing He, Yunyun Yue, Qinxin Zhang, Shuang Wang, Yunfeng Zhang, Yuxuan Wei, Qingshun Zhao

Open access · goldAbstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nan Li *Model Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Dongya Jiang *Model Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Luqingqing HeModel Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Yunyun YueSchool of Traditional Chinese Pharmacy, China Pharmaceutical University, NanjingJiangsu, 210009, China.
Qinxin ZhangDepartment of Prenatal Diagnosis, Obstetrics and Gynecology Hospital, Affiliated to Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, 210004, Jiangsu, China.
Shuang WangModel Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Yunfeng ZhangModel Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Yuxuan WeiModel Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.
Qingshun ZhaoModel Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China. qingshun@nju.edu.cn.ORCID http://orcid.org/0000-0001-9536-4672
Model Animal Research Center · CNChina Pharmaceutical University · CNNanjing Maternity and Child Health Care Hospital · CN

Funding

National Natural Science Foundation of China 31970769
6 · The paper itself

Abstract

In the evolutionary "arms race" from prokaryotes to eukaryotes, some memories of foreign DNA have been conserved for defensive purposes. Shortly after invasion by the plasmid, pEGFP-N1, the conserved the defense gene, isg15, was activated in the zebrafish zygote and in mammalian cells. Based on the sequence similarity, we found three virus-derived sequences in pEGFP-N1 which share the 5'-GTTTGTT-3' core sequence, an epigenetic factor leading to increased expression of isg15. Mutation of the core sequence greatly reduces the degradation rate of the plasmid in E. coli cells or zebrafish embryos. We conclude that a conserved defense response, common to both eukaryotic and prokaryotic cells, allows identification and degradation of plasmids containing 5'-GTTTGTT-3'.

Indexed as

5ʹ-GTTTGTT-3ʹCore sequenceDefense responseEukaryotic cellForeign plasmidInnate immune memoryISG15Prokaryotic cellTransformation efficiency

Identifiers

PMID35690839
PMCPMC9188160
OpenAlexW4282912934

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.