Evidence map›Paper›PMID 35689713›Full record

ArticleAMB Express2022

Detection of low-load Epstein-Barr virus in blood samples by enriched recombinase aided amplification assay.

Jing-Yi Li, Xiao-Ping Chen, Yan-Qing Tie, Xiu-Li Sun, Rui-Qing Zhang, An-Na He, Ming-Zhu Nie, Guo-Hao Fan, Feng-Yu Li, Feng-Yu Tian and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Observational
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Jing-Yi Li *Hebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China.
Xiao-Ping Chen *State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China.
Yan-Qing Tie *Hebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China.
Xiu-Li SunHebei General Hospital, No. 348 West Heping Road, Shijiazhuang, 050070, Hebei, China.
Rui-Qing ZhangNHC Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China.
An-Na HeNHC Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China.
Ming-Zhu NieHebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China.
Guo-Hao FanNHC Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China.
Feng-Yu LiHebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China.
Feng-Yu TianHebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China.
Xin-Xin ShenNHC Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China. x616815@126.com.
Zhi-Shan FengHebei Medical University, No. 361 East Zhongshan Road, Shijiazhuang, 050031, Hebei, China. 15131129999@139.com.
Xue-Jun MaNHC Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, No. 155, Changbai Street, Changping District, Beijing, 102206, China. maxj@ivdc.chinacdc.cn.ORCID http://orcid.org/0000-0001-5891-5260
Hebei Medical University · CNNational Institute for Viral Disease Control and Prevention · CNNorth China University of Science and Technology · CNNational Institute for Communicable Disease Control and Prevention · CN

Funding

IVDC 2019HYDQNJJ03the key R & D projects in zibo city 2020kj100011
6 · The paper itself

Abstract

Epstein-Barr virus (EBV), a common human γ-herpesvirus, infects more than 90% of adults worldwide. The purpose of this study was to establish a novel EBV detection method by combining the recombinase aided amplification (RAA) assay with an initial enrichment step that utilizes magnetic beads coated with a recombinant human mannan-binding lectin (rhMBL, M1 protein). An M1 protein-protein A magnetic bead complex (M1 beads) was prepared and used to achieve separation and enrichment of EBV from blood. After nucleic acid extraction, DNA was amplified by RAA. Using 388 whole blood samples and 1 serum sample, we explored the specificity, sensitivity and applicability of the newly developed detection method and compared it with commercial quantitative real-time polymerase chain reaction (qPCR) following M1 bead enrichment, traditional qPCR and traditional RAA. After enrichment, the positivity rate of EBV was increased from 15.94% to 17.74% by RAA (P < 0.05) and from 7.20% to 15.17% by qPCR (P < 0.05). The viral loads after enrichment were increased by 1.13 to 23.19-fold (P < 0.05). Our data demonstrates that an RAA assay incorporating M1 bead enrichment is a promising tool for detecting low EBV viral loads in blood samples that will facilitate an early response to EBV infection.

Indexed as

EnrichmentEpstein-Barr virusLow viral loadM1 beadsRAASensitive detection

Identifiers

PMID35689713
PMCPMC9188631
OpenAlexW4282928252

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.