ArticleNature communications2022
Imaging translational control by Argonaute with single-molecule resolution in live cells.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 37 citations in OpenAlex.
- Translation of a small upstream open reading frame functions as a rheostat for the regulation ofbioRxiv : the preprint server for biology · 2026Article
- Quantitative comparison of methodologies for translation site imaging in living cells.RNA (New York, N.Y.) · 2026Article
- Live-cell co-translational folding tracking reveals bidirectional coupling between translation and folding.bioRxiv : the preprint server for biology · 2026Article
- Bridging single-molecule and genome-wide studies of cellular mRNA translation.RNA (New York, N.Y.) · 2026Review
- MicroLive: an image processing toolkit for quantifying live-cell single-molecule microscopy.Bioinformatics advances · 2026Article
- Systematic characterization of the composition and dynamics of processing body-associated mRNAs.Nature communications · 2025Article
- MicroLive: An Image Processing Toolkit for Quantifying Live-cell Single-Molecule Microscopy.bioRxiv : the preprint server for biology · 2025Article
- FTO-mediated mClinical and translational medicine · 2025Article
- All Probes Plasmids (APPs) for multicolor and long-term tracking of single-mRNA translation dynamics.Molecular biology of the cell · 2025Article
- Expanding the tagging toolbox for visualizing translation live.The Biochemical journal · 2025Review
- A platform for analyzing translational control by RBPs at single-mRNA resolution in cells.Biophysics and physicobiology · 2025Article
- Imaging and Quantifying Ribosomal Frameshifting Dynamics with Single-RNA Precision in Live Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Inorganic arsenic modulates cell apoptosis by regulating Argonaute 2 expression via the p53 pathway.Toxicology research · 2025Article
- Exploring the dynamics of messenger ribonucleoprotein-mediated translation repression.Biochemical Society transactions · 2024Review
- RNA granules in flux: dynamics to balance physiology and pathology.Nature reviews. Neuroscience · 2024Review
- Translation Dynamics of Single mRNAs in Live Cells.Annual review of biophysics · 2024Review
- A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies.Nature communications · 2024Article
- Synonymous codon usage regulates translation initiation.Cell reports · 2023Article
- microRNAs in action: biogenesis, function and regulation.Nature reviews. Genetics · 2023Review
- The EDC4-XRN1 interaction controls P-body dynamics to link mRNA decapping with decay.The EMBO journal · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A major challenge to our understanding of translational control has been deconvolving the individual impact specific regulatory factors have on the complex dynamics of mRNA translation. MicroRNAs (miRNAs), for example, guide Argonaute and associated proteins to target mRNAs, where they direct gene silencing in multiple ways that are not well understood. To better deconvolve these dynamics, we have developed technology to directly visualize and quantify the impact of human Argonaute2 (Ago2) on the translation and subcellular localization of individual reporter mRNAs in living cells. We show that our combined translation and Ago2 tethering sensor reflects endogenous miRNA-mediated gene silencing. Using the sensor, we find that Ago2 association leads to progressive silencing of translation at individual mRNA. Silencing was occasionally interrupted by brief bursts of translational activity and took 3-4 times longer than a single round of translation, consistent with a gradual increase in the inhibition of translation initiation. At later time points, Ago2-tethered mRNAs cluster and coalesce with P-bodies, where a translationally silent state is maintained. These results provide a framework for exploring miRNA-mediated gene regulation in live cells at the single-molecule level. Furthermore, our tethering-based, single-molecule reporter system will likely have wide-ranging application in studying RNA-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.