Evidence map›Paper›PMID 35687262›Full record

ArticleBiomolecular NMR assignments2022

ILV methyl NMR resonance assignments of the 81 kDa E. coli β-clamp.

Socheata Lim, Sam Mahdi, Penny J Beuning, Dmitry M Korzhnev

Abstract read
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In one paragraph

Article in Biomolecular NMR assignments, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Socheata LimDepartment of Molecular Biology and Biophysics, University of Connecticut Health Center, 06030, Farmington, CT, USA.
Sam MahdiDepartment of Molecular Biology and Biophysics, University of Connecticut Health Center, 06030, Farmington, CT, USA.
Penny J BeuningDepartment of Chemistry and Chemical Biology, Northeastern University, 02115, Boston, MA, USA.ORCID 0000-0002-7770-022X
Dmitry M KorzhnevDepartment of Molecular Biology and Biophysics, University of Connecticut Health Center, 06030, Farmington, CT, USA. korzhniev@uchc.edu.ORCID 0000-0002-3556-2447

Funding

Dynamics of Processivity Clamp Proteins in Bacterial DNA ReplicationR01GM123239 · NIGMS · NORTHEASTERN UNIVERSITY · PI BEUNING, PENNY J, KORZHNEV, DMITRY M. · 2017 to 2021
$1.8M
NIGMS NIH HHS R01 GM123239NIH HHS R01GM123239
6 · The paper itself

Abstract

The ring-shaped E. coli β-clamp protein is an 81 kDa head-to-tail homodimer, which serves as a processivity factor anchoring the replicative polymerase to DNA, thereby increasing replication processivity and speed. In addition, it facilitates numerous protein transactions that take place on DNA during replication, repair, and damage response. We used a structure-based approach to obtain nearly complete Ile, Leu and Val side-chain methyl NMR resonance assignments of the wild-type β-clamp and its stabilized T45R/S107R variant based on site directed mutagenesis and the analysis of methyl-methyl NOESY data. The obtained assignments will facilitate future studies of the β-clamp interactions and dynamics.

Indexed as

Escherichia coliEscherichia coli ProteinsDNAMutagenesisNuclear Magnetic Resonance, BiomolecularDNAEscherichia coli ProteinsBacterial DNA replicationDnaNPol III holoenzymeProcessivity β-clamp

Identifiers

PMID35687262
PMCPMC10752501

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.