Strategies for Multienzyme Assemblies.

Qing Sun, Maryam Raeeszadeh-Sarmazdeh, Shen-Long Tsai, Wilfred Chen

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Qing Sun *Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.

Maryam Raeeszadeh-Sarmazdeh *Department of Chemical and Materials Engineering, University of Nevada, Reno, NV, USA.

Shen-Long Tsai *Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan.

Wilfred ChenDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA. wilfred@udel.edu.

Funding

Virtual Reality and Augmented Reality CoreP20GM103650 · NIGMS · UNIVERSITY OF NEVADA RENO · PI WEBSTER, MICHAEL A · 2012 to 2022

$20.9M

NIGMS NIH HHS P20 GM103650

6 · The paper itself

Abstract

Proteins are not designed to be standalone entities and must coordinate their collective action for optimum performance. Nature has developed through evolution the ability to co-localize the functional partners of a cascade enzymatic reaction in order to ensure efficient exchange of intermediates. Inspired by these natural designs, synthetic scaffolds have been created to enhance the overall biological pathway performance. In this chapter, we describe several DNA- and protein-based scaffold approaches to assemble artificial enzyme cascades for a wide range of applications. We highlight the key benefits and drawbacks of these approaches to provide insights on how to choose the appropriate scaffold for different cascade systems.

Indexed as

DNAProteinsDNAProteinsBioconjugationEnzyme CascadeMetabolonProtein AssemblySubstrate Channeling

Identifiers

PMID35687232

DOI10.1016/j.copbio.2015.08.009

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.