Evidence map›Paper›PMID 35687227›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Multi-dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells.

Songon An, Prakash Parajuli, Erin L Kennedy, Minjoung Kyoung

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Songon AnDepartment of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), Baltimore, USA. san@umbc.edu.
Prakash ParajuliDepartment of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), Baltimore, USA.
Erin L KennedyDepartment of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), Baltimore, USA.
Minjoung KyoungDepartment of Chemistry and Biochemistry, University of Maryland Baltimore County (UMBC), Baltimore, USA. mjkyoung@umbc.edu.
University of Maryland, Baltimore County · US

Funding

EXPAND PARTICIPATION BY MINORITIES IN BIOMEDICAL SCIENCER25GM055036 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI SUMMERS, MICHAEL FINLEY · 1996 to 2021
$16.9M
Graduate Training at The Chemistry Biology InterfaceT32GM066706 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI SELEY-RADTKE, KATHERINE L, SMITH, AARON T · 2004 to 2023
$3.3M
4D functional mapping of glucose metabolism in Living CellsR01GM134086 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI KYOUNG, MINJOUNG · 2019 to 2023
$1.6M
A Multienzyme Metabolic Complex for Glucose MetabolismR01GM125981 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI AN, SONGON · 2018 to 2022
$1.4M
Functional Contribution of Metabolic Complex to Cancer Cell MetabolismR03CA219609 · NCI · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI AN, SONGON · 2018 to 2019
$127k
NCI NIH HHS R03 CA219609NIGMS NIH HHS R01 GM125981NIGMS NIH HHS R01 GM134086NIGMS NIH HHS R25 GM055036NIGMS NIH HHS T32 GM066706
6 · The paper itself

Abstract

Fluorescence live-cell imaging that has contributed to our understanding of cell biology is now at the frontline of studying quantitative biochemistry in a cell. Particularly, technological advancements of fluorescence live-cell imaging and associated strategies in recent years have allowed us to discover various subcellular macromolecular assemblies in living human cells. Here we describe how real-time dynamics of a multienzyme metabolic assembly, the "glucosome," that is responsible for regulating glucose flux at subcellular levels, has been investigated in both 2- and 3-dimensional space of single human cells. We envision that such multi-dimensional fluorescence live-cell imaging will continue to revolutionize our understanding of how intracellular metabolic pathways and their network are functionally orchestrated at single-cell levels.

Indexed as

GlucoseImaging, Three-DimensionalHumansMicroscopy, FluorescenceGlucoseCancer MetabolismFluorescence MicroscopyGlucose MetabolismGlucosomeGlycolysisLattice Light-sheet MicroscopyLive-cell ImagingMacromolecular AssemblyMetabolic ComplexMetabolic Condensate

Identifiers

PMID35687227
PMCPMC9191769
OpenAlexW4285203576

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.