Evidence map›Paper›PMID 35686656›Full record

ArticleJournal of applied microbiology2022

A novel phage-displayed MilA ELISA for detection of antibodies against Myc. bovis in bovine milk.

Mina Farzaneh, Abdollah Derakhshandeh, Abd Al-Bar Ahmed Al-Farha, Kiro Petrovski, Farhid Hemmatzadeh

Abstract read
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Article in Journal of applied microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Frontiers in veterinary science · 2024
    Review
  3. Bacteriophage Therapy to Control Bovine Mastitis: A Review.Antibiotics (Basel, Switzerland) · 2023
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mina FarzanehDepartment of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Abdollah DerakhshandehDepartment of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.ORCID https://orcid.org/0000-0002-1878-1103
Abd Al-Bar Ahmed Al-FarhaDepartment of Animal Production, Technical Agricultural College, Northern Technical University, Mosul, Iraq.
Kiro PetrovskiAustralian Centre for Antimicrobial Resistance Ecology, The University of Adelaide, School of Animal and Veterinary Sciences, South Australia, Australia.
Farhid HemmatzadehDavies Research Centre, School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia.ORCID https://orcid.org/0000-0002-4572-8869

Funding

School of Veterinary Science, Shiraz University 0GCB1M163973
6 · The paper itself

Abstract

aimsThe aim of this study was to assess a phage-displayed MilA protein of Myc. bovis in an indirect ELISA for the detection of Myc. bovis antibodies in milk samples. METHODS AND

resultsThe desired sequence of milA gene was synthesized and cloned into pCANTAB-F12 phagemid vector. The expression of the MilA on the phage surface was confirmed by Western blotting. The recombinant phage was used in the development of an indirect ELISA to detect Myc. bovis antibodies in milk samples. There was a significant agreement between the results of phage-based ELISA and recombinant GST-MilA ELISA for the detection of Myc. bovis antibodies in milk samples.

conclusionsThe inexpensive and convenient phage-based ELISA can be used instead of recombinant protein/peptide ELISA as an initial screening of Myc. bovis-associated mastitis. SIGNIFICANCE AND IMPACT OF STUDY: Mastitis associated with Myc. bovis is a continuous and serious problem in the dairy industry. Sero-monitoring of Myc. bovis infection cases are one of the key factors for surveillance of the infections in dairy farms. Despite the existence of some commercially serological assays for Myc. bovis antibodies, they have some limitations regarding their sensitivity and availability. The development of accurate diagnosis tools could contribute to control programmes of Myc. bovis-associated mastitis in the dairy herds.

Indexed as

BacteriophagesMastitisMycoplasma bovisMycoplasma InfectionsAnimalsEnzyme-Linked Immunosorbent AssayFemaleHumansMilkRecombinant ProteinsRecombinant Proteinsindirect ELISAMilA antigenMilkMyc. bovisphage display

Identifiers

PMID35686656
PMCPMC9545076

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.