ArticlePolymers2022
Polyhydroxyalkanoates from a Mixed Microbial Culture: Extraction Optimization and Polymer Characterization.
Article in Polymers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 42 citations in OpenAlex.
- Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates.Microbial biotechnology · 2026Article
- Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse UsingPolymers · 2026Article
- Isolation and Purification of Bacterially Produced Polyhydroxyalkanoates: Mechanisms, Limitations, and Current Advances.Life (Basel, Switzerland) · 2026Review
- Upcycling agricultural residues into polyhydroxyalkanoates through pilot-scale mixed microbial culture processes.Frontiers in bioengineering and biotechnology · 2026Article
- Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement.Scientific reports · 2025Article
- Article
- Biosynthesis of Polyhydroalkanoates Doped with Silver Nanoparticles UsingInternational journal of molecular sciences · 2024Article
- A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater.Microbial cell factories · 2024Review
- Article
- Can Biomass Mastication Assist the Downstreaming of Polyhydroxyalkanoates Produced from Mixed Microbial Cultures?Molecules (Basel, Switzerland) · 2023Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Polyhydroxyalkanoates (PHA) are biopolymers with potential to replace conventional oil-based plastics. However, PHA high production costs limit their scope of commercial applications. Downstream processing is currently the major cost factor for PHA production but one of the least investigated aspects of the PHA production chain. In this study, the extraction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced at pilot scale by a mixed microbial culture was performed using sodium hydroxide (NaOH) or sodium hypochlorite (NaClO) as digestion agents of non-PHA cellular mass. Optimal conditions for digestion with NaOH (0.3 M, 4.8 h) and NaClO (9.0%, 3.4 h) resulted in polymers with a PHA purity and recovery of ca. 100%, in the case of the former and ca. 99% and 90%, respectively, in the case of the latter. These methods presented higher PHA recoveries than extraction by soxhlet with chloroform, the benchmark protocol for PHA extraction. The polymers extracted by the three methods presented similar PHA purities, molecular weights and polydispersity indices. Using the optimized conditions for NaOH and NaClO digestions, this study analyzed the effect of the initial intracellular PHA content (40-70%), biomass concentration (20-100 g/L) and biomass pre-treatment (fresh vs. dried vs. lyophilized) on the performance of PHA extraction by these two methods.
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