Three dimensional reconstruction of the mouse cerebellum in Hedgehog-driven medulloblastoma models to identify Norrin-dependent effects on preneoplasia.

Nenad T Pokrajac, Akshay Gurdita, Nobuhiko Tachibana, Nicholas J A Tokarew, Valerie A Wallace

Open access · goldAbstract read

In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.3field-weighted citation impact, top 49% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Nenad T PokrajacDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, M5T 2S8, Canada.ORCID 0000-0002-0548-779X

Akshay GurditaDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, M5T 2S8, Canada.

Nobuhiko TachibanaDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, M5T 2S8, Canada.

Nicholas J A TokarewDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, M5T 2S8, Canada.

Valerie A WallaceDonald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, Toronto, ON, M5T 2S8, Canada. valerie.wallace@uhnresearch.ca.

University Health Network · CA

Funding

CIHR

6 · The paper itself

Abstract

Spontaneous mouse models of medulloblastoma (MB) offer a tractable system to study malignant progression in the brain. Mouse Sonic Hedgehog (Shh)-MB tumours first appear at postnatal stages as preneoplastic changes on the surface of the cerebellum, the external granule layer (EGL). Here we compared traditional histology and 3DISCO tissue clearing in combination with light sheet fluorescence microscopy (LSFM) to identify and quantify preneoplastic changes induced by disrupting stromal Norrin/Frizzled 4 (Fzd4) signalling, a potent tumour inhibitory signal in two mouse models of spontaneous Shh-MB. We show that 3DISCO-LSFM is as accurate as traditional histology for detecting Norrin/Fzd4-associated changes in PNL formation in Ptch

Indexed as

Cerebellar NeoplasmsMedulloblastomaAnimalsCerebellumDisease Models, AnimalHedgehog ProteinsImaging, Three-DimensionalMiceHedgehog Proteins

Identifiers

PMID35680976

DOI10.1038/s42003-022-03507-5

PMCPMC9184598

OpenAlexW4281613358

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.